aThe polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from DNA polymerase. PCR is used to amplify a piece of DNA by in vitro enzymatic replication. It uses repeated cycles, each of which consists of three steps: 聚合酶链式反应(PCR)是技术...
PCRtechniqueDNAThe polymerase chain reaction (PCR) is a technique commonly used by molecular biologists to make additional copies of DNA, which may be used for a variety of purposes (see pp. 38-41).Benjamin EvansLaura EmeryBiological sciences review...
Rapinese, A. DiIorio, E. Toniato, and S. Martinotti, "Molecular approach by PCR is the best method to detect the presence of Chlamydia trachomatis and to define the true agent of ocular bacterial inflammation," International Journal of Immunopathology and Pharmacology, vol. 24, pp. 285-296,...
and PCR-based ampilficati.on of specific genetic regions. The resulting DNA fragments are then ligated to technology-specific adaptor sequences, forming a fragment library. These adaptors may also have a unique molecular “barcode”, so ...
Mullis, was awarded the Nobel Prize in Chemistry in 1993, is often hailed as one of the most important scientific achievements in molecular biology. The polymerase chain reaction (PCR) is a typical laboratory technique for making multiple copies of a specific area of DNA (millions or billions!
Molecular response assessed in pb using a PCR technique is, along with beta 2M, the most important factor to predict FFS in FL. 展开 关键词: BCL-2, follicular lymphoma minimal residual disease PCR prognostic factors DOI: 10.1093/annonc/11.suppl_1.S137 被引量: 133 ...
sequencing, analysis, and experimentation—especially when an initial sample is too small to utilize by itself. Since its discovery in 1984, PCR has become fundamental to the field of microbiology. It is a simple technique with myriad applications, from criminal forensics to the detection of ...
4c, d). Subsequently, to understand the regulation exerted bymiR-188-3p over TMED3, variation of mRNA of TMED3 was detected using qRT-PCR technique after forced or abrogated expression of miR-188-3p. It was observed that TMED3 mRNA was drastically decreased with forced expression of miR-...
Another molecular subtyping technique called multiple locus variable number tandem repeat analysis (MLVA) has also been used to determine precise classification when it is difficult to differentiate between isolates with common PFGE patterns (https://www.cdc.gov/pulsenet/p...). PFGE and PulseNet ...
These factors include: accurate identification of the microorganisms used as the starting material for the postbiotic; description of the inactivation procedure or technique, as each process can result in a different postbiotic composition with different effects; and a description and quantification of ...