After permeabilization, embryos were washed three times in PBS-T (0.1% Tween in PBS), free aldehydes were removed by short incubation in NH4Cl (2.6 mg ml−1) and the embryos were washed twice in PBS-T. The embryos were blocked and incubated with anti-CRABP2 antibody, then washed...
adult males were maintained for a month to ensure sufficient maturation of the glands. Both the left and right organs were excised together with fine forceps (#5 Dumont) and spring scissors (#15002-08 FST) in phosphate-buffered saline (PBS) (Fig.3A). After fixation...
A new investigation by National Public Radio (NPR) and the Public Broadcasting Service (PBS)reports that the large oil and gas companies that manufacture plastics have known for decades that recycling plastic was unlikely to ever happen on a broad scale because of the-high costs involved. "They...
The samples were diluted with PBS to ensure equal loading volumes and heated at 70 °C for 10 min. Samples were run on 4–12% NuPAGE Bis-Tris gradient mini gels (Life Technologies; Grand Island, NY) at 150 V using MOPS buffer (50 mM MOPS, 50 mM Tris, 0.1% SDS, 1 mM EDTA; pH...
Cell colonies were washed with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde, stained with 0.1% crystal violet and imaged using an optical microscope. TUNEL assay and flow cytometry To detect apoptosis in sections of tumor tissues, a TUNEL assay was performed according to the ...
The cells were harvested in ice-cold PBS and lysed with lysis buffer (150 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and cOmplete protease inhibitor cocktail (Roche)). 90% of each cell lysate was centrifuged at 13,000 rpm for 5 minutes and the supernatants were transferred and...
were then placed in 1 mL of the staining solution for 75 min. Thereafter, the embryos were washed for 30 s with 60% isopropanol and then rinsed again for 3 min in 60% isopropanol, followed by a 30-s wash in 1% PBS. Next, the ORO stain was extracted from the embryos for ...
On the day of staining, after rehydration for 4 min in 0.02 M PBS (pH 7.4), sections were incubated for 60 min at 4 °C with ALPase reaction medium, which contained 0,05MTris-HCl (pH 8.5), 5 mL; Fast Blue BB Salt hemi (zinc chloride) salt (Sigma-Aldrich, Milwaukee, WI, USA)...
This sample was obtained by inserting standard absorbent papers (Periopapers, Oral Flow®, Plainview, NY, USA) into the periodontal sulcus for 30 s and vortexing them for 10 s to elute the Periopaper content into PBS before transferring them to polypropylene tubes with thioglycolate medium. ...
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