In tree shrews, recombinant CYP2E1 protein and liver microsomes metabolised chlorzoxazone and p-nitrophenol, probe substrates of human CYP2E1, just as they do in dogs and pigs.4. These results suggest that tree shrew CYP2E1 encodes a functional drug-metabolising enzyme that plays a role in ...
What is the pKa of the deprotonated p-nitrophenol? What is an epoxide? Describe the action of a buffer composed of citric acid (H3C6H5O7) and sodium citrate (NaH2C6H5O7) Which of the following combinations will not produce a buffer solution? (a) H N O 2 and N a N O 2 (b)...
Enzyme activity. The enzyme activity of PE11 was measured, as described elsewhere45, using different esters of p-nitrophenol (pNP) (Sigma-Aldrich, USA) such as acetate (pNPC2), butyrate (pNPC4), octanoate (pNPC8), laurate (pNPC12), myristate (pNPC14), palimitate (pNPC16) and ...
After completion of the incubation period, 100 mM sodium carbonate solution was added to the reaction mixture and the concentration of p-nitrophenol was quantified by measuring the absorbance at 420 nm. To assay activity towards recalcitrant substrates, the incubation time was extended from 30...
Nitrobenzene exposure can be estimated from p-nitrophenol concentration in the urine or methemoglobin levels in the blood. Exposure to organophosphorus insecticides can be estimated from measurements of plasma or erythrocyte cholinesterase activity. biological oxygen demand (BOD; biochemical oxygen demand) ...
Alkaline phosphatase activity in rat serum was measured using an end-point colorimetric spectroscopy (Sigma-Aldrich, St. Louis, MO). The procedure was modified to accommodate the small volume of samples available. Enzymatic activity was determined by measuring the release of p-nitrophenol from substra...
Explain why p-nitrophenol is a stronger acid than phenol itself. Why is ethanoic acid considered an acid? Why is aspirin less polar than caffeine? Explain why some substances are soluble in water and some are not. Why is aspirin more polar than salicylic acid?
Moreover, it is a typical enzyme used as a marker for osteoblast identifica- tion in vitro. Osteoblasts plated in triplicate were washed twice with PBS. Afterwards, 500 μL p-nitrophenyl phos- phate (pNPP) buffer (0.2% 4-nitrophenyl-phosphate diso- dium salt hexahydrate, 50 mM glycine, 1...
Released p-nitrophenol was measured at 405 nm. α-Amylase and β-amylase activity were determined with the CERALPHA and BETAMYL kits respectively (Megazyme, Ireland). Limit dextrinase activity was assayed at 37 °C in assays containing 20 mM sodium acetate, pH 5.5, 5 mM ...
The activity of alkaline phosphatase (AP) is analyzed by the hydrolysis of p-nitrophenyl phosphate into phosphate and p-nitrophenol, which are measurable through photometry. Ferritin levels are determined by an ElektroChemiLumineszenz ImmunoAssay that uses ferritin-specific antibodies conjugated with ...