aOne unit of enzyme activity was defined as the amount of enzyme required to release 1 nM p-nitrophenol per minute at 50 ?C, pH 5.0. 酶活性一个单位被定义了,酵素要求的数量发布每分钟1毫微米p亚硝基苯酚在50 ?C,酸碱度5.0。 [translate] aThe standard package of system documentation will consist...
To estimate the strength of interactions, β-galactosidase activity was measured after growth of theE. colistrains in liquid medium in the presence of IPTG and antibiotics, usingο-nitrophenol-β-galactoside as a substrate. Theο-nitrophenol produced per mg of protein versus time was represented, a...
PnP Purine Nucleoside Phosphorylase (enzyme) PnP Policía Nacional del Perú PnP Positive Negative Positive PnP Peer Networking Protocol PnP Pneumoperitoneum PnP p-Nitrophenol PnP Paper and Pencil PnP Propylene Glycol N-Propyl Ether PnP Policy and Procedure PnP Plug N Pray PnP Party and Play PnP Priv...
Although the insecticides parathion and diazinon are well distributed throughout the body and extensively bind to both plasma and tissue proteins, they are both rapidly cleared from the body primarily in the urine as degradation metabolites of the parent compounds [i.e., p-nitrophenol, 2-isopropyl...
In tree shrews, recombinant CYP2E1 protein and liver microsomes metabolised chlorzoxazone and p-nitrophenol, probe substrates of human CYP2E1, just as they do in dogs and pigs.4. These results suggest that tree shrew CYP2E1 encodes a functional drug-metabolising enzyme that plays a role in ...
Enzyme activity. The enzyme activity of PE11 was measured, as described elsewhere45, using different esters of p-nitrophenol (pNP) (Sigma-Aldrich, USA) such as acetate (pNPC2), butyrate (pNPC4), octanoate (pNPC8), laurate (pNPC12), myristate (pNPC14), palimitate (pNPC16) and ...
TG1/pBS(Kan)T3MO produced 66% p-nitrophenol and 34% m-nitrophenol from nitrobenzene and 100% p-methoxyphenol from methoxybenzene, as well as 62% 1-naphthol and 38% 2-naphthol from naphthalene; similar results were found with TG1/pBS(Kan)T4MO. Sequencing of the tbu locus from pBS(Kan)T3...
Table 5. Metabolism of fenitrothion by isolated P. clarkii hepatopancreas cells incubated for 12 hours with 5 μM fenitrothion. Values are mean ± SD (n = 3) range. Empty CellFenitrothion (nmol)Fenitrooxon (nmol)3-methyl-4-nitrophenol (nmol) killed cells* 5.60±0.78 n.d. ad. live ce...
(1984) Comparison of p-nitrophenol biodegradation in field and laboratory test systems. Appl. Environ. Microbiol. 48, 944–950. CAS Google Scholar Steber, J. and Wierich, P. (1989) The environmental fate of fatty acid a-sulfomethyl esters. TensideSurfact. Deterg. 26, 406–411. CAS ...
Enzyme assays Plate assay Rhodamine B agar plates were used for the detection of lipolytic activity from microbial strains. Rhodamine B agar plates (20 ml volume) were prepared using the method described by Kouker and Jaeger 1987). Spectrophotometric assay p-NPP (para--Nitrophenol Palmitate) was...