Here we show that the RNA-binding protein and ribonuclease polynucleotide phosphorylase (PNPase) is a positive regulator of biofilm formation in the human pathogen Listeria monocytogenes, a major responsible for food contamination in food-processing environments. The PNPase mutant strain produces less ...
Among these proteins, heat shock protein 90 (FgHsp90) was revealed to play a central role in heat shock stress responses in this fungus. FgHsp90 was highly expressed and exclusively localised to nuclei in response to heat stress. Moreover, our comprehensive functional characterisation of FgHsp...
An enzyme that digests protein in the small intestine...its from pancreas? You have developed a genetically engineered bacterial strain that can make extremely large amounts of human catalase, but so much so that the catalase becomes an insoluble aggregate and has...
CUTL1, also known as CDP (CCAAT displacement protein), Cut, or Cux-1, belongs to a family of homeobox transcription factors involved in the regulation of cell growth and differentiation (Nepveu, 2001). It is evolutionarily conserved and contains four DNA binding domains, three of which are ...
Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Mol. Cell Biol. 15, 3917–3925 (1995). 27. Nathan, D. F., Vos, M. H. & Lindquist, S. Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp...
Lowest Km with highest substrate (ortho-nitrophenyl-β-galactoside or ONPG) affinity was measured in presence of Ca2+ (42.45 μM ONPG). However, our results demonstrated that Vmax was maximum in presence of Mn+2 (55.98 μM ONP produced mg?1 protein min?1), followed by Fe+2, Zn+2, ...
50 μL of chloroform was added, and solutions were vortexed for 15 sec and incubated at 37 °C in a water bath for 5 min. 200 μL of pre-warmed ONPG (4 mg/mL) was added to the solution. The reaction was stopped by adding 500 μL of 1 M NaCO3 when the ...
At least three individual clones for each variant were tested for liquid b-galactosidase assays using ONPG,22 and the assays were performed in triplicate. Activity was determined as a comparison to wild-type BRCA1 and S1613G (positive controls) or to M1775R, and Y1853X (negative controls)....
This hypothesis was supported by genetic analysis revealing that ∆mxdB mutants were unable to transition from a cell monolayer to a three dimensional biofilm structure [4]. MxdC shares homology with an efflux pump and mxdD was annotated as a conserved hypothetical protein with no known homology...
Using bacterial-two hybrid assays, we showed thatYycH andYycI interact, and thatYycHI also interacts with the sensor kinase WalK, forming a ternary protein complex. Mutation toYycH orYycI associated with clinical VISA strains had a deleterious impact on theYycHI/WalK complex, suggesting that ...