From a cDNA library, a nucleotide sequence of a novel gene, called rig, specifically expressed by streptozotocin- or alloxan-nicotinamide-induced rat insulinomas was determined and an amino acid sequence of a protein encoded by the gene was deduced. Further, novel genes with base sequences ...
as well as discovery and analysis of different types of genomic features in a single sequencing run, from single nucleotide variants (SNVs), to copy number and structural variants, and even RNA fusions. NGS enables an ideal throughput per run, and studie...
In the field of biology, the term “sequencing” refers to the determination of the sequence of units of a linear polymer. DNA sequencing, therefore, is the use of methods and technologies to determine the identity and order of the four nucleotide bases (adenine, guanine, cytosine, and thymin...
Lsr2 is a nucleoid-associated protein conserved in mycobacteria and actinomycetes encoded by thelsr2gene24.M. tuberculosisLsr2 is composed of two domains: an N-terminal DNA-binding domain that binds preferentially to AT-rich DNA and a C-terminal oligomerization domain that promotes nucleoprotein fil...
This is confirmed by the chromatogram in c, which shows that the 12-nucleotide deletion scrambles downstream reading of the nucleotide sequence in NGS protocols. d The mutant protein is expressed, as seen in immunoprecipitation-coupled mass spectrometry. A peak corresponding to a protein with a ...
which were specific to Pinb gene in cereal crop.There existed large differences of sequences of Pinb between Ae.juvenalis and Triticum aestivum.Compared with Pinb-D1a from T.aestivum cv.Capitole,the Pinb genes in Ae.juvenalis shared 93.1% and 93.3% homologies in nucleotide sequence and ...
DNA is made up of molecules called nucleotides. Each nucleotide contains three components: a phosphate group, which is one phosphorus atom bonded to four oxygen atoms; a sugar molecule; and anitrogenbase. The four types of nitrogen bases are adenine (A), thymine (T), guanine (G) and cytos...
From the results of the nucleotide sequences from 5′-RACE experiments and ESTs, we predicted the whole sequence of the mRNA molecule. Using primers consistent with 5′-part and 3′-part sequences of the predicted molecule, a 2.3-kb-length cDNA was amplified from total RNA of HEL cells ...
. These two MAGs have an average nucleotide identity (ANI) of 98.9%, confirming that the Gakkel Ridge plumes host two closely related strains of the sameSulfurimonasspecies. The 16S rRNA gene sequences from the MAGs are 99.5−100% identical with the dominant OTUs of the 16S rRNA gene ...
(2018). An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein inter- actions. PLoS One 13, e0208273. https://doi.org/10.1371/journal.pone. 0208273. Melhem, H., Spalinger, M.R., Cosin-Roger...