(57) cDNA encoding [summary] mammal, the AMP protein kinase, recombinant protein with an antibody against the polypeptide as well as their corresponding. Their use, including the characterization of proteins and other test of gene expression.ラジンダークマール...
The purified protein has an intrinsic nucleoside triphosphatase activity which is independent of, and not stimulated by, any common nucleic acid cofactors. All eight common ribo- and deoxyribonucleoside triphosphates are hydrolyzed to the diphosphate form in the presence of a divalent cation. ...
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase...
of the disorder-mediated control of the exposure of protein–protein interfaces, here we argue that an analogous mechanism can pertain to protein–DNA interface exposure at the level of the nucleosome and show that modulating DNA access through histone tails might represent a rather general mechanism...
Lactococcus lactis, the well-known Gram-positive lactic acid bacteria used in the food industry, have recently gained attention as a prospective therapeutic. L. lactis strains have been shown to be a promising host for heterologous protein expression [43,44,45] and for the production of native ...
This section provides a quick introduction of the 5 primary nucleobases: Adenine (A), Cytosine (C), Guanine (G), Thymine (T) and Uracil (U).
Sequence analysis suggests that YhhN proteins contain eight transmembrane helices, an N-in/C-in topology, and about 5 highly conserved amino acid residues that may form an active site. This work is the first to demonstrate a function for a bacterial YhhN protein, as a vinyl ether bond ...
The fusion protein in ISAV is synthesized as a precursor protein designated as F0. In order to exert its biological function, F0 must be cleaved by cellular proteases to generate F1 and F2 [17]. Several insertions of 10-12 amino acids (IN1-4), identical to sequences from other genomic...
Proteins with a metallo-beta-lactamase (MBL) fold have been largely studied in bacteria in the framework of resistance to beta-lactams, but their spectrum of activities is broader. We show here that the giant Tupanvirus also encodes a MBL fold-protein that has orthologs in other giant viruses...
Biotin-labeled DNA was further detected by chemiluminescence using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific). In vitro DNA elongation assay A 99-nt DNA oligo was used as the template. The template-primer complex was prepared in the following reaction, including ...