A series of isoxazolidines with various alkyl substituents at N and an ethoxy group at C(5) have been synthesised and their NMR spectra recorded over a range of temperatures. The NMR spectra at low temperature indicate the presence of two isomers due to slow nitrogen inversion. The nitrogen ...
Nitrogen-15 NMR chemical shift tensors and conformation of some nitrogen-15-labeled polypeptides in the solid state Not Available A Shoji,T Ozaki,T Fujito,... - 《Macromolecules》 被引量: 57发表: 1989年 Application of infrared laser to the zebrafish vascular system: gene induction, tracing,...
Finally, the solvent was removed under a stream of nitrogen and the ODL was re-dissolved in acetone (10 μg/μl) for in vivo 13C labelling. Gene Cloning. The coding-sequences of the target genes25 were amplified from cDNA-containing plasmid constructs (synthesised by GeneArt...
Chemical reaction kinetics provide a quantitative or qualitative measurement of the rate(s) of reactions and provide insight into the dependence of these rates on variables such as concentration, temperature, pressure, the presence of catalysts, the physical state of the reactants, etc. Since chemic...
This is mediated by the catalytic lysine (K83) and the active-site water installed with the E105G mutation. Supplementary information Supplementary Information Supplementary Tables 1–4, Supplementary Methods, Supplementary Notes 1–3, enzyme DNA sequences and NMR spectra. Reporting Summary ...
The 13C nuclear magnetic resonance (NMR) data of this compound showed signals of two carbonyl carbons at δC 180.6 and 177.9. A strong absorption band at 1634 cm−1 in the infrared (IR) data indicated these to be amide carbonyls. Then, the presence of a single nitrogen inherent in the...
Proton NMR (sometimes low temperature NMR or NOE) is the major reliable method for conformational analysis of isoxazolidines 〈82JOC4397, 84OMR(22)399〉. It is generally agreed that conformers created by isoxazolidine derivatives are promoted by the pyramidal inversion of the ring nitrogen...
Note:Cooling the bath with liquid nitrogen works similarly to the above steps, but requires active stirring after coolant addition. LN2 has a tendency to freeze the top layer of the bath, and this must be broken up and stirred into the liquid fraction. ...
The task of efficiently reducing harmful nitrogen oxides (NOx) emissions from low-temperature diesel exhausts during engine cold starts remains demanding. Passive NOx adsorbers (PNA), offering the capability of temporarily trapping NOx at low temperatures (below 200°C) and releasing the captured NOx...
(a) The arginine demethylation reaction of KDM6B requires both 2OG and Fe(II) for active demethylation. The degree of arginine demethylation of H3(14–34)K27Rme2a catalysed by KDM6B was quantified by MALDI-TOF mass spectrometry. Data show the mean±s.e.m. (b) 1H NMR analyses of KDM...