In general, sporulation may be triggered by nutritional stress, nitrogen or carbon scarcity, quorum sensing, or other unknown signals [22,52]. The lack of nutrients results in lower intracellular GTP concentrations, the proximal signal for sporulation. Sporulation is an irreversible high-cost process...
Embryos used for ChIP-seq experiments were flash frozen in liquid nitrogen and stored at −80 °C. For ATAC-seq and scRNA-seq experiments, the embryos were processed immediately after dechorionation without crosslinking. For immunostainings, antibodies were used in the following dilutions: ...
Nitrogen, and more particularly hydrogen, have very low solubilities so that it is difficult to assess their roles: likewise, grown-in carbon concentrations are usually very small in modern crystals. Nevertheless, progress in our understanding continues and processes relating to hydrogen are now ...
Developing xylem was harvested from a 10 cm long stem segment at the bottom (Figure 1). The surface of debarked wood was scraped with a razor blade as described previously [37],[38]. The samples, which consisted of a soft mush of tissue, were shock-frozen in liquid nitrogen and stored...
ofPoritesspp. toPorites lobata. The genotyping of colonies of a subset of 11 islands confirmed that the sampled colonies are phylogenetically related to the targeted species71,77. Samples were flash-frozen in liquid nitrogen on board and kept stored at −20 °C before DNA extraction and ...
[2]Some examples of rare skills/focuses:high nitrogen compounds,high speed photography, metal-organic frameworksandcrystallography, and (my focus) whole-cell biochemistry. In some fields the barrier to entry is technical. If your work requires a really expensive piece of equipment, only a few lab...
In addition, pathways related to ‘Nitrogen metabolism’, ‘Starch and sucrose metabolism’, and ‘Photosynthesis-antenna proteins’ seem to actively participate in C. breviculmis shade tolerance. Plants have evolved a complex sucrose transport reaction to avoid shade stress [36]. Sucrose phosphate ...
However, the upregulation of the expression of these two mitochondrial TDP-dependent enzymes can result not only from the increased requirement for mitochondrial potential restoration, respiration control, nitrogen metabolism or glutamate signaling [30] but also reflect the activation of a stress ...
In a healthy adult, the endogenous microbially produced gut gases (hydrogen, methane, and insignificant quantities of odorogenic sulfides and ammonia) are only a minor amount as opposed to bulk of gases, predominantly oxygen, nitrogen, and carbon dioxide, generated every day from exogenous air ...
corrosive medium. Since the OHB has a sizable molecular structure and contains many heterogeneous atoms (four nitrogen atoms in addition to one oxygen and one sulfur atom), it is believed that the binding of OHB molecules to the mild steel surface is responsible for the strong inhibition ...