Although MTHFD2 primarily localizes within the mitochondria, its nuclear23and chromatin43localizations have been previously reported in some cancer cell lines. Therefore, we asked whether MTHFD2 chromatin localization is a generalizable event. We selected a panel of breast, colon and lung cancer cell ...
(Δ166-324), ΔC-term (Δ677-864) or ΔMid-DR (Δ325-676) (cKO-CENP-C/GFP-CENP-C ΔCBD, ΔC-term, ΔMid-DR)37 were re-established in this study by transfection of a plasmid encoding GFP-fused CENP-C ΔCBD, ΔC-term or ΔMid-DR and a neomycin resistance gene into cKO-...
HEK293FT cells in DMEM were supplemented with 10% fetal bovine serum and 100 units/mL penicillin, streptomycin, and neomycin, and 293F cells in 293 Freestyle media were transfected with plasmids using Lipofectamine 2000 (Invitrogen). Adenovirus containing JMJD1C, sh-JMJD1C, and JMJD1C mutants...
Two days after transfection ESC were selected with Neomycin (200 μg/mL) for one additional week. Individual ESC clones were picked and amplified based on eGFP expression indicating successful CTCF targeting. HTI and western blot analyses were used to verify that GFP and CTCF were lost upon ...
The cellular transmembrane protein CD317/BST-2/HM1.24/Tetherin restricts HIV-1 infection by physically tethering mature virions to the surface of infected cells. HIV-1 counteracts this restriction by expressing the accessory protein Vpu, yet the mechanis
Recent experiments show that adaptive Darwinian evolution in one environment can lead to the emergence of multiple new traits that provide no immediate benefit in this environment. Such latent non-adaptive traits, however, can become adaptive in future e
MDCK and HepG2 cells were transfected by the standard lipofectamine (Thermo Fisher) protocol. Stable cell lines were obtained by selection of transfected cultures with neomycin during 10 days. The cells were cultured as previously described [41]. Briefly, cells were incubated in Dulbecco's ...
The Emerson family moves to a California beach town, but soon realize something is not quite right with their new surroundings. Young Sam Emerson (Corey Haim) is warned of the local vampire population by a pair of odd siblings known as the Frog brothers. The more vocal of the brothers, ...
KLHL14 is a substrate-binding subunit of Cullin-RING ligase 3 ubiquitin ligase complex, highly enriched in thyroid since early embryonic development, together with its antisense RNA KLHL14-AS. We have previously demonstrated that Klhl14-AS is a competing
The neomycin-resistant cassette was removed by crossing with transgenic mice expressing FLPe under the CAG promoter and this allowed the generation of mice with the conditional floxed (fl or tm1b) allele (Canxfl/+ or Calrfl/+). These mice were crossed with transgenic mice expressing Cre under...