•While the word "alkaline" means "basic," "alkali" and "base" are not synonymous. An alkali, in fact, is one of a specific group of elements on the periodic table, as you'll soon learn. So be aware of the alkali vs. alkaline distinction. An example of a strong base is sodium ...
In an alkaline fuel cell, the electrolyte is an alkaline solution, often potassium hydroxide (KOH) or sodium hydroxide (NaOH). The alkaline electrolyte allows the conduction of hydroxide ions (OH-) between the fuel and oxidant electrodes, enabling the electrochemical reaction to occur. ...
Nucleotide exchange from GDP to GPPNHP (Jena Biosciences) was performed before the final gel-filtration purification and according to a published procedure comprising EDTA-mediated exchange, desalting and cleavage of residual GDP with an alkaline phosphatase (CIP or Quick CIP; New England Biolabs)2,...
NaOH soln. and an aq. NaOCl soln., are appropriately used as the alkaline soln. The soln. of Ru and Os is distilled by a distillation device furnished with an Ru absorbing HC trap and an Os absorbing aq. NaOH soln. trap. As a result, the Ru is absorbed in the HCl. The HCl soln...
The sequential extracts in alkaline media (NaOH) (Hernández-Garibay et al., 2011; Magnusson et al., 2019) indicate that the configuration of the isolated ulvan molecules influence the immune response; thus, unaltered ulvan is required for successful stimulation of Solea macrophages by Ulva ohnoi...
For Rpb1 degradation, whole cell extracts were prepared by alkaline (denaturing) extraction (Kushnirov, 2000). In order to visualize ubiquitylated yeast Rpb1, the total ubiquitylated protein pool was isolated from whole cell extracts prepared by bead-beating in lysis buffer (150 mM Tris-acetate ...
The reaction products were fractionated by electrophoresis on an alkaline agarose gel (0.8%) containing 30 mM NaOH. The gels were analyzed with a FluoroImager 595 (GE Healthcare). In vitro exonuclease reaction. The DNA substrates used in the exonuclease reaction were prepared as follows. ...
Maximum gel strength (Nikan, 954g cm 2 ) was obtained with 5% NaOH at 100°C after 90min of treatment, though these conditions resulted in an agar yield reduction of 25.5% relative to native agar. This treatment proved to efficiently yield G. lemaneiformis agar that will meet the ...
Amino acids 133–458 are annotated as an alkaline phytoceramidase domain by PANTHER. (d) Real-time RT–PCR of TOD1 expression pattern in different tissues of Arabidopsis. Data are mean±s.e. (n=4). (e–h) Cell-specific pTOD1::gTOD1-GUS expression. (e) Mature pollen grain, (f) ...
(THERMOSCIENTIFIC) at 4 °C overnight and the reaction mix was used to transform competent DH5-alpha cells. The recombinant plasmids were isolated using the standard alkaline lysis method and sequenced to confirm their appropriate integration in the vector. Mussel receptors were not cloned in ...