Are nonmetals reactive? Is lawrencium a metal? What is sodium chloride? Is salt an amorphous solid? Is sodium fluoride ionic, covalent, or polar covalent? Is vanadium a metal, nonmetal or metalloid? Is NaCl organic or inorganic? What is the atomic mass of sodium chloride? What is between...
by atomic absorption spectroscopy. A typical polar solvent is aq. NaCl (0.5-2.0 wt.%; 15 cm3), and a typical non-polar solvent is thin liquid paraffin (pharmaceutical quality) or olive oil (15 cm3).doi:EP0633470 A2HALBACH STEFAN DR.EP...
The native and SeMet-labeled complex of Krr1(32–222) and a Faf1 fragment containing residues 145–169 and 199–220 were crystallized at 20 °C using the hanging-drop vapor diffusion method by mixing 1 μl of protein (20 mg ml−1 in 10 mm Tris-HCl, pH 8.0, and 300 mm NaCl) an...
The Michaelis constant (Km) value was assayed in a buffer at final volume of 40 µL (100 mM HEPES, pH 7.4;100 mM NaCl; 2 mM MgCl2; 0.1 mM DTT; 0.5 mM EDTA; and 1 mg/mL BSA) in a 384-well format. A series of different substrate concentrations (NAD+ from 0 mM to 5 mM, ...
The disc vesicles were subsequently washed three times to remove excess hydroxy- lamine and retinaloxime and resuspended in buffer C (10 mM HEPES pH 8.0, 100 mM NaCl, 300 mM sucrose, 1 mM MgCl2) for the retinal transfer assays. Retinoid transfer from disc vesicles to acceptor ...
Polar auxin transport is required in the blue light regulation of LeEXT expression In order to check the independence of auxin and blue light transduction pathways in the regulation of the steady state level of LeEXT mRNAs, we studied the role of auxin transport in this photo- regulation. ...
HisHasA from a soluble fraction of a 500-ml culture was loaded on a Q-Sepharose column (10 ml) and eluted with a 0 to 1 m NaCl gradient in 50 mm Tris-HCl at pH 7.5, allowing for separation of heme-loaded from heme-free protein. The heme-free protein-containing fractions were then...
For resealing, 50 mL of 53 resealing buffer (750 mM NaCl, 25 mM Na2HPO4, pH 7.4) was carefully added to the RBC suspension and incubated for 60 min at 37C while gently rocking. Preloaded RBCs were washed three times in cRPMI and ...
To determine the preferred O2concentration, WC8-2 was grown in Basal_YE (pH 6.0, 30 °C, no NaCl) in butyl stopper-sealed glass bottles with the atmosphere in the headspace adjusted to different N2/O2/CO2ratios (70:20:10%, 80:10:10%, 89:1:10%, 90:0:10% v/v) after removing...
Amphiphilic additives improve drug solubility in water by trapping molecules and forming nanoscale aggregates; micelles35,36. DMSO and NMP are polar solvents. The addition of Kolliphor®HS 15, an amphiphilic nonionic surfactant formerly known as Solutol HS 15, to DMSO dramatically increased RFB solu...