The mechanism of solubility depends on the solute and solvent. Describe on an ionic or molecular scale how each of the following dissolve: a. NaCl in H2O b. I2 in CCl4 c. CH3CH2OH in H2O Explain how temperature affects the solubility of an ionic ...
What is the molecular weight of NaCl? How do you calculate it? How many grams of NaCl, molecular mass = 58.5 Daltons, are the molar equivalent to 50 g of glucose (molecular mass = 180 Daltons)? Solution: 0.9% NaCl a) Demonstrate how to convert the n...
After loading, the column was washed with 30 ml of the same buffer and then eluted with a linear gradient (0.1-1.0 M) of NaCl in 42 ml of the 10 mM HEPES-NaOH (pH 6.5) buffer. Size exclusion chromatography Two types of columns were used: Superdex 75 10/300 GL and Superdex 200 10...
Edge length of NaCl (a) = 2(rNa++rCl−)=2×(2.814×10−8×1010pm)=562.8 No. of atoms per unit cell (Z) = 4 Density of NaCl (ρ)=2.167 g cm−3 Gram molecular mass of NaCl (M) = 58.45 g mol−1 N0=4×(58.45g mol−1)(562.8)3×(2×167 g cm−3)×(10...
Cells were allowed to express for 12 h, then collected by centrifugation and resuspended in 180 ml HEPES pH 7.5, 1 mM DTT, 0.5 mM EDTA, 20% glycerol and 400 mM NaCl. Purification of Rad24-RFC Cells were lysed by a French Press using three passes at 22,000 psi for...
Storage buffer: 40 mM HEPES, 150 mM NaCl, 4 mM MgCl2, 5% glycerol, 7% D2O. Titrated to pH 7.4 with NaOH. Preparation of 15N-labeled KRAS(G12C)-MRTX849 A 4-ml 0.10 mM sample of U-15N KRAS(G12C)−GDP 1−169 (Mr = 19,579) was reserved before the final gel...
A 1/10 volume of proteinase K (1 mg/mL) in proteinase K buffer (10 mM Tris-Cl pH8.0, 25 mM EDTA, 100 mM NaCl,0.5% SDS) was added to the media, and incubate at 55 °C for 4 h with rotation. An equal volume of Phenol: Chloroform: Isoamyl Alcohol (Thermo Fisher)...
A variety of reagents was used in efforts to remove the vitelline membrane of the eggs of Spisula solidissima. Alkaline, isotonic NaCl was found to remove the membrane, but only after germinal vesicle breakdown subsequent to fertilization (except on long-term treatment). The process of membrane...
Is a solid produced by mixing a solution of NaCl (aq) and KNO3 (aq)? a. No b. Yes, AgCl (s) c. Yes, NaNO3 (s)Double-Replacement Reactions:A double-replacement reaction is a reaction in which the cations and anions of the reactants are swit...
Why is HCl called a polar molecule? Explain your reasoning. Why does the heat of dissolution of NaCl need to be considered when NaCl is being used as a deicer? Why does the molten sodium chloride conduct electricity, but the solid form of sodium chloride does not?