P-glycoprotein (Pgp) is an ABC transporter responsible for the ATP-dependent efflux of chemotherapeutic compounds from multidrug resistant cancer cells. Better understanding of the molecular mechanism of Pgp-mediated transport could promote rational drug
which is accepted by OGT, resulting in the installation of O-GlcNDAz moieties onto native substrates78. Brief UV treatment of GlcNDAz-labeled cells affords the covalent, carbene-mediated, in situ
We aimed to identify the main native molecule(s) responsible for this effect and to elucidate its mechanism of action. Materials and methods Bacterial strains, media, and growth conditions In the study, we tested three Lactococcus lactis spp. lactis strains (Lc2, Lc3, Lc4), obtained from the...
[36]. recently, it was shown that clpp is the molecular target of the small molecule tic10 (onc201) [19,41], currently in phase iii clinical trials for glioma treatment. detalied biochemical and crytalography data shows that tic10 binds to clpp via non-covalent allosteric interactions, ...
It was suggested that Apg16 forms an oligomer through the coiled-coil region and functions as a linker to form the Apg12-Apg5·Apg16 multimeric complex (16). Because Apg16 is the only molecule thus far identified to interact with the Apg12-Apg5 conjugate, further characterization of Apg16...
Is the solid state of {eq}SiO_2 {/eq} classified as an ionic crystal, a covalent crystal, a molecular crystal, or a metallic crystal? Explain. Solid-state Crystals: The solid state crystals would have a definite arrangement of constituent...
epithelial tissue. Mucus was diluted in a 1:1 (v/v) ratio with either 6 M GuHCl solution (denatured preparation) or 0.2 M NaCl solution (native preparation) for 24 h at 4 °C. The NaCl solution contained 0.04% sodium azide, 0.1 M EDTA, 1 M aminohexanoic acid, 0....
16. The method of claim 1, wherein the compound is a peptidomimetic. 17. The method of claim 1, wherein the compound is an organic molecule. 18. The method of claim 1, wherein the compound is a polyepetide, a nucleic acid, or an inorganic chemical. 19. The method of claim ...
The first strategy, in which the native entanglement is stabilized by drug binding, failed to decrease misfolding because it formed an alternative entanglement at a nearby region. The second strategy, in which a small molecule was designed to bind to a non-native tertiary structure and thereby ...
each strand of a DNA molecule is the complement of the other. The process by which two complementary DNA strands associate with one another is termed hybridization. The process of strand separation, which is generally accomplished by heating the sample, is termed melting or denaturation of the du...