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Further purification was performed using an anion exchange column, HiTrap SP Fast Flow (GE Healthcare, 17-5054-01). The eluted proteins were loaded onto a HiLoadTM 16/600 SuperdexTM 75 pg (GE Healthcare, 28-9893-33) gel filtration column equilibrated with a buffer containing 40 mM ...
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DAF-FM-DA fluorescence was imaged using an Andor Revolution WD (with Borealis) spinning-disk confocal imaging system (Andor Technology, Belfast, UK), consisting of an upright Nikon microscope with a water immersion objective (60× magnification, numerical aperture [NA] 1.0) and an electron-...
Role of Cation Size in the Energy of Electron Transfer to 1:1 Polyoxometalate Ion Pairs {(M+)(Xn+VW11O40)}(8-n)- (M = Li, Na, K) Computational and experimental studies were performed to determine the role of N(3)H hydrogen bonding in controlling flavin redox chemistry. B3LYP calcula...
In this study, the putative anion transporter 1 (ANTR1) from Arabidopsis thaliana was shown to be localized to the chloroplast thylakoid membrane by Western blotting with two different peptide-specific antibodies. ANTR1 is homologous to the type I of mammalian Na(+)-dependent inorganic phosphate ...
A cholesterol level depressant containing a non-cross-linking anion exchange resin having a structural unit represented by general formula (I) as the main ingredient, wherein R1 represents benzyl or C1-C20 alkyl; R2 and R3 may be the same or different from each other and each represents C1-...
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