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Further purification was performed using an anion exchange column, HiTrap SP Fast Flow (GE Healthcare, 17-5054-01). The eluted proteins were loaded onto a HiLoadTM 16/600 SuperdexTM 75 pg (GE Healthcare, 28-9893-33) gel filtration column equilibrated with a buffer containing 40 mM ...
DAF-FM-DA fluorescence was imaged using an Andor Revolution WD (with Borealis) spinning-disk confocal imaging system (Andor Technology, Belfast, UK), consisting of an upright Nikon microscope with a water immersion objective (60× magnification, numerical aperture [NA] 1.0) and an electron-...
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Role of Cation Size in the Energy of Electron Transfer to 1:1 Polyoxometalate Ion Pairs {(M+)(Xn+VW11O40)}(8-n)- (M = Li, Na, K) Computational and experimental studies were performed to determine the role of N(3)H hydrogen bonding in controlling flavin redox chemistry. B3LYP calcula...
An unprecedented trimer based on monovacant Dawson anion: [(α2-P2W17O61)Ln(H2O)4]321− (Ln = LaIII, CeIII and PrIII) Three unprecedented trimers based on monovacant Dawson-type polyoxotungstates Na12[Ln(H2O)9]2[(α2-P2W17O61H4)La(H2O)4]6·100H2O (Ln = LaIII (1)) and ...
A cholesterol level depressant containing a non-cross-linking anion exchange resin having a structural unit represented by general formula (I) as the main ingredient, wherein R1 represents benzyl or C1-C20 alkyl; R2 and R3 may be the same or different from each other and each represents C1-...
Serum anion gap (AG) is closely related to mortality in critically ill patients with several diseases. We aimed to determine the relationship between serum AG levels and 28-day intensive care unit (ICU) mortality in patients with diastolic heart failure (DHF). This cohort study enrolled ...
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