Crh proteins catalyze crosslinking of chitin and glucan polymers in fungal cell walls. Here, we show that the BcCrh1 protein from the phytopathogenic fungus Botrytis cinerea acts as a cytoplasmic effector and e
highly conserved across the four species; thus our experimental design facilitates an analysis of binding attributable to differences in the genome sequence or nuclear environment between species, rather than to the Sox proteins themselves (Additional file1: Figure S1). We used a differential enrichment...
generated by qPCR; and statistics were performed using unpaired T test.dAmount of aerobic colony forming units (CFU) per gram of feces on bothLactobacillus-selective (MRS) and general growth (TSA) media.S. hispidusdisplayed a higher amount of aerobic growth using both methods. Significance was ...
L. acidophilusor identified FARM strains were inoculated into 2 ml of MRS broth and incubated. At the end of the exponential growth phase, the cell density was estimated again by measuring the absorbance
Is MacConkey agar a selective medium or a differential medium? How do you know? 1. Define a differential medium. What is its purpose? 2. Define a selective medium. What are its uses? 3. Why is MacConkey agar selective as well as differential? 4. Why ...
agar plates [55,56] and incubated at 37 °C until single colonies could be picked — this took between 24 to 96 h depending on the strain. Single colonies were picked and used to inoculate 20-mL fresh YCFA or MRS broth. Broth was incubated at 37 °C until turbid. MRS broth was ...
E.coli strains were grown on LB broth agar or in LB broth, Mycobacterium smegmatis mc2155 was grown in 7H9 liquid medium (Difco) supplemented with 0.05% w/v Tween 80, 0.5%glycerol and 0.5%glucose or were grown on 7H10 agar supplemented with 1% glycerol and 0.5% glucose. The starvation...
a P2X7 antagonist, but this compound lacked selectivity, limiting its use as a specific P2X7 antagonist. Nevertheless, given this broad inhibitory action against P2 receptors, the possibility remains that other amiloride or HMA derivatives may be identified or developed as more selective P2X or P2Y...