DEPC isgood for RNA related work, otherwise you can use Milli Q from water purification systems attached such as DNae/RNase filter membrane. ... Use RNase inhibitors like RNasin for reactions involving RNA, and DEPC treated sterile water to rinse equipments. That is what we practice in our ...
loliiformis were washed thrice with sterile MilliQ water followed by surface sterilization with 70% ethanol for 30s with vortexing and re-washing for 3 times with MilliQ water to get rid of traces of ethanol. The grains were then soaked in 6.5% sodium hypochlorite (w/v) for 5min and ...
Approximately 600 female STDR pupae were injected in the abdomen with 1.3 μg/μl of Nv-tra2 dsRNA, Nv-tra dsRNA or, as a negative control, sterile milliQ water. Nv-tra was used as a positive control (cf. Verhulst et al., 2010a). Injections were performed with Femtotips II (Eppend...
To compensate for water evaporation 700 µL of sterile MilliQ water was added to the cultures on a daily basis (measured by weight difference). For the assessment of biomass accumulation at 5% (v/v) CO2 using both MAD and MAD2 media, 1–2 µL of sterile Antifoam 204 (Sigma-...
sterile permeable membrane (Air-O-Seal; 4titude) that allows good gas exchange and humidity between the bacteria in the medium and the jar atmosphere. The microplates were incubated in hermetic jars filled with 4.5% (v/v) MilliQ sterile water. MIC values were read after 24 h of incubation...
sterile needle tip before passing through an 18-gauge syringe and centrifuging for 2 min at 12,000×g. In the final step, RNA was eluted in 50 μL mqH2O and stored at − 20 °C. RNA was purified using ethanol precipitation. The RNA pellet was resuspended in 15 μL milliq H2O ...
Parent-specific methylation of specific CpG residues is critical to imprinting in eutherian mammals, but its importance to imprinting in marsupials and, thus, the evolutionary origins of the imprinting mechanism have been the subject of controversy. This has been particularly true for the imprinted Ins...
(Bioline) and 66.8 µL sterile MilliQ water. The PCR amplifications were made with an initial denaturation step at 95 °C for 1 min, followed by 30 cycles of denaturation at 95 °C for 1 min, annealing for 1 min at 55 °C and an extension at 72 °C for 1 min; following these...
Water-soluble metabolites were extracted from peat by adding 7 ml of autoclaved milliQ water to 1 g of wet peat in a sterile 15 ml centrifuge tube. Tubes were vortexed twice for 30 s, and then the peat–water mixture was sonicated for 2 h at 22 °C. Samples were then ...
washed thoroughly through several passages in sterile Volvic water and then kept in the last water aliquot overnight to reduce the load of random bacteria present in food vacuoles. Then stentors were washed briefly in autoclaved sterile distilled water, in order to reduce contaminants and microorganis...