Cells (1 × 107 cells) grown on a 10-cm dish were trypsinized and washed twice with PBS and once with 10 ml of buffer A (10 mM Tris-HCl pH 7.4, 8.3 mM KCl, 1.3 mM NaCl, 1.5 mM MgSO4). After each step, the cells were sedimented (10 minutes, 660 × g) and finally resuspend...
(a,b) Immunodetection of OmpX (a) and Ail (b) inY.enterocoliticaparental strain JB580v andhfqmutant SOR17 grown for 4 h in LB. Upper panels show immunoblots, and lower panels show part of the gels stained for total proteins. (v), vector pACYC184ts; (phfq), plasmid phfq. Percenta...
. The slices were washed three times and then incubated for 60 minutes to allow metabolic recovery in a modified Krebs-Henseleit buffer (124 mmol/L NaCl, 5 mmol/L KCl, 1.2 mmol/L KH2PO4, 1.2 mmol/L MgSO4, 26 mmol/L NaHCO3, 5 mmol/L glucose, and 1.2 mmol/L CaCl2).29 After ...
SOC medium (0.5% yeast extract, 2% tryptone,10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, 20 mM glucose) was used to recover bacteria after electroporation and prior to plating. For production of the Myf fimbriae, Y. enterocolitica strains were grown in Porcine Brain ...
How many grams of MgSO4 (mM= 120 g/mol) must be added to 147 grams of water to make a solution with a colligative molality of 0.250 m? What is the molar concentration of 30 g KCl and 30 g of CaCl2 added to 50 dL of DI water?
The scientific study of matter's characteristics and behaviour is known as chemistry.Chemistry vocabularyandChemistry glossaryare different from the normal vocabulary. It is a branch of natural science that examines the building blocks of matter, including the atoms, molecules, and ions that make up...
Cells were then kept at room temperature for additional 10 min in a physiological solution containing 140 NaCl, 3.6 KCl, 0.5 NaH2PO4·H2O, 0.5 MgSO4·7H2O, 1.5 CaCl2·2H2O, 10 HEPES, 3 glucose (in mM), and 0.1% BSA (pH 7.4). Nominally Ca2+-free medium was prepared by addition of ...
There's more to your question that it may seem, but it has nothing to do with life. Although it is usually said that a molecule is ghe smallest part of a compound that keeps its properties, this is too simplistic. All therdominamical properties of a compound (enthalpy, entropy, boiling...
The hippocampus was dissected free within ice-cold Krebs' solution composed of (mM): NaCl 124; KCl 3; NaH2PO4 1.25; NaHCO3 26; MgSO4 1; CaCl2 2; and glucose 10, previously gassed with 95% O2 and 5% CO2, pH 7.4. Slices (400 mm thick) were cut perpendicularly to the long axis of...
Overnight cultures were grown from a single colony in K10 media consisting of 46 mM Na2HPO4, 23 mM NaH2PO4, 8 mM (NH4)2SO4, 10 0.4 mM MgSO4, 3 mM thiamine, mM KCl and 100 6 mM iron, 0.04% mM NaCl, supplemented glucose plus 100 mg ml À 1 with ampicillin. The next morning, ...