Nitric oxide inactivates methionine adenosyltransferase viaS-nitrosylation of cysteine 121. Replacement of the acidic (aspartate 355) or basic (arginine 357 and arginine 363) amino acids located in the vicinity of cysteine 121 by serine leads to a marked reduction in the ability of nitric oxide ...
The cells were subsequently washed with cold acidic buffer (0.2 M 100% acetic acid, 0.5 M, NaCl, pH 4.2) three times to strip surface-residing transferrin and then washed with phosphate-buffered saline and fixed. Transferrin internalization was measured as fluorescence intensity of ...
Recognizing that OLs detect axonal spiking, we next asked whether heightened axonal activity influences metabolic flux within OLs. Considering that OLs may supply axons with glycolytic products such as pyruvate or lactate, increased axonal spiking could enhance glucose consumption in OLs. To study this,...
1998). The respective criteria were: (i) the isoelectric pH values of the keratin that determines its classification as acidic, type I and basic to neutral, type II cytokeratin; (ii) its relative molecular mass on SDS–polyacrylamide gels, which defines the type-specific numerical designation ...
H3K4me3 and H3K9me3 are unstable in methionine- addicted cancer cells, but stable in normal cells [9]. High expression of H4K20me3 was associated with good prognosis in colon cancer [10]. Therefore, the catalytic enzymes of these non-permanent epigenetic markers are of particular interest in...
What functional groups are found in the side groups of acidic amino acids? What functional groups are found in the side groups of basic amino acids? Which of the following amino acid combinations have side chains with groups that have the greatest ability to stabilize the tertiary structure...
The pKa of histidine is 6.0, so histidine is best at buffering at pH 6.0. The acidic amino acids have pKa's below histidine's, and the basic amino acids have
The effects of dietary protein content on diurnal variations in plasma concentrations of neutral and basic amino acids, and on red blood cell levels of acidic amino acids, were studied in seven normal humans. The subjects consumed, on three consecutive 3-day periods, diets containing 0, 75, or...
(pH = 9.4) buffer. Acidic conditions could facilitate attachment of triptonide to the beads via the ketone or possibly the lactone, leaving unhindered epoxide groups, whereas basic conditions may promote binding via one of the epoxide groups. We, therefore, used both acidic and basic ...
Sera were purified using SulfoLink Coupling Resin (Thermo Scientific), according to the manufacturer's protocol, using His-ISCA2 or the respective epitope-peptide. Antibodies were eluted in acidic conditions (0.1 M glycine, pH 2.8). Antibody-containing fractions were then dialysed against PBS and ...