85. Briefly, water extracts were acidified to pH 2 using 1 M HCl. Then, extracts were filtered through a 3 ml Bond Elut PPE cartridge (Agilent) that was previously activated using methanol. Cartridges were washed with 3 ml of a 0.01 M HCl solution five times, then dried using...
The dried fractions were dissolved in methanol and loaded with the reference standard AFB1 on the TLC plate, which was then developed in a solvent system composed of toluene: ethyl acetate: formic acid (6:3:1, v:v:v). When the solvent system reached the end of the plate by 2 cm, ...
As seen for other similar compounds, hydrogen bond is formed between the hydroxyl group of the 55c methanol moiety and the oxygen of the phosphate group. The preferential binding selectivity of 55c for intercalation in AT-rich regions is probably attributable to steric hindrance between the...
Whole-mountin situhybridization (WISH), was carried out as previously described29on embryos fixed overnight in 4% paraformaldehyde/phosphate buffered saline (PBS), then rinsed with PBS-Tween (0.1% Tween-20), dehydrated in 100% methanol and stored at −20 °C until processed for WISH30.rl...
propanoic acid and methanol C. ethanoic acid and propanol D. methanoic acid and propano Redox reactions do not need water to occur. Indeed, there is no reason why a reaction may not be both a decomposition reaction and a re...
C) Acidic oxide. D) Hydrogen gas. E) Lithium peroxide. A certain substance contains only C, H, and S. Combustion of a 5.00 g sample in excess oxygen yields 4.67 g of carbon dioxide and 2.87 g of water and an unknown amount of sulfur trioxide. What are t...
have recently not only confirmed the effect of deuterium content in water on the function of mitochondria, but also concluded that this effect is because of water itself rather than alterations in biomolecular mechanics of the cells (57). If this latter result is confirmed, the exact molecular ...
Desertification is ridding ourselves of valuable, life-giving soil. Pesticides used for destroying insects or other organisms that are harmful to cultivated plants, may contain ammonia, benzene, chlorine, dioxins, ethylene oxide, formaldehyde and methanol. These substances become persistent soil contaminant...
Mature seeds (2 g) separated from pods were finely powdered and extracted with 120 mL of methanol for 5–6 h followed by concentration in vacuo at 40 °C. 25 mL of 15 % methanolic-HCl was added and reflux for 2 h. Acidic solvent was removed under vacuum to dryness. Residue was extra...
Wash weak and strong weak solutions are composed of Heptane/Isopropanol (90:10, v/v) and Isopropanol/Methanol/Chloroform (45:45:10, v/v/v) respectively. Finally, the make-up solution is composed of ACN. MS data were acquired in negative ionization mode on a Xevo TQ-MS triple quadrupole...