maintained in position throughout the 90 min of addition. The stirred reaction mixture is thengradually allowed to warm to room temperature over the next 8–10 hr. The reaction iscarefully quenched at room temperature by dropwise addition of 1 : 1 water : acetic acid (ca.20 mL) with stirri...
Cells (5000–10,000) were plated on 60-mm dishes and incubated at 37 °C, 5% CO2 for colony formation. After 7 days, colonies were fixed with 10% (v/v) methanol for 15 min and stained with 5% Giemsa (Sigma, St. Louis, USA) for 30 min for colony visualization. Enzymatic ...
Briefly, water-extracted metabolites were thawed at room temperature and centrifuged again to remove any particles that potentially formed after thawing. Next, each sample was split into two 2 ml glass tube vials (1 ml each), one for hydrophilic interaction liquid chromatography (HILIC) and ...
1) Methane is the simplest hydrocarbon and is a colourless and odourless gas at room temperature and pressure. Methane is also known as marsh gas or methyl hydride. While storing in the cylinders for usage and transportation, methane is mixed with odorant, tert-butylthiol, to find out the l...
摘要: A nuclear reactor fuel element component (25) comprises a zirconium alloy, and has an oxide layer (27) which is formed on the component surface by oxidation in a hydrogen-free gas atmosphere at increased temperature.收藏 引用 批量引用 报错 分享 ...
This is equal to what temperature in Celsius and Kelvin? The boiling point of methanol is 65 degrees Celsius, and the boiling point of ethanol is 78 degrees Celsius. Which of the following statements is true? A) At 70 degrees Celsius, you would have met...
The stock solutions were then used to prepare a working solution of 50 ng/mL in methanol containing all compounds. FTOHs stock solutions of 4:2 FTOH, 6:2 FTOH, 8:2 FTOH, and 10:2 FTOH were also bought separately at 50 µg/mL in methanol and used to prepare a working solution at...
Briefly, samples (1–5 μg protein) in 50 mm (NH4)HCO3, pH 8.0, were vacuum-dried, dissolved in 6 m guanidine HCl followed by the addition of 20 mm DTT, and incubated for 1 h at room temperature. Thiols were blocked with 100 mm iodoacetamide for 1 h in the dark, and the sample...
Age and sex are major risk factors in Alzheimer’s disease (AD) with a higher incidence of the disease in females. Neuroinflammation, which is a hallmark of AD, contributes to disease pathogenesis and is inexorably linked with inappropriate microglial ac
a Nitrite-induced optical changes to deoxyferrous alpha globin caused by NO gas formation and binding are reflected in the changing spectra over time. Sodium nitrite solution (1 mM) was added to deoxyferrous alpha globin (4 μΜ), and spectra were recorded at 10-s intervals. b Example...