MacConkey agar is a selective and differential culture medium. It is selective because it contains crystal violet and bile salts which inhibit the growth of Gram-positive bacteria, therefore selecting for the growth of Gram-negative bacteria. It is also differential bec...
What is the application (purpose) of MacConkey agar? Why is a vas deferens longer than an oviduct? What are the 20 variables that may affect seed germination? What is the purpose of the soft agar? Explain. What is a seed and what are its three major components?
A 1:1 v/v broth culture containing 1×108colony-forming unit/mL of each test isolate (donor) and sodium azide–resistantE.coliJ53 (recipient) was mated overnight at 37 °C in nutrient broth (NB), and transconjugants were selected on MacConkey agar containing COL (4 g/mL) and sodium ...
Utilizing membrane filters with decreasing pore sizes, 500 mL aliquots were filtered to allow water passage and trap bacteria. Optical immunosensor From each specimen, the resultant filters were distributed across two modified MacConkey (MAC) broths; one contained 0.05 grams per milliliter of meropenem...
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The strains were grown statically at 37 °C for 16 hours in LB broth supplemented with 50 mg/l kanamycin and the optical densities (OD600nm) were standardized to contain 9.2 log10 CFU/ml, which was confirmed retrospectively by plating 10-fold serial dilutions on MacConkey agar. Groups ...
After a 3-h incubation, unbound bacteria were removed and EPEC adherent to HT29 cells were quantified by CFU determinations on MacConkey agar. Results are expressed as mean Ϯ SE of at least three experiments run in triplicate. Adherence of EPEC was decreased in wells containing either M-SAA...
Stool samples were harvested on day 12 and plated on MacConkey agar (Remel) for evaluation of C. rodentium fecal load. DHR oxidation assay on murine neutrophils Production of neutrophil-derived ROS was measured using a DHR oxidation assay. Room temperature heparinized blood (300 μL) obtained ...
Lactose-containing MacConkey agar (Difco) was supplemented with appropriate antibiotics when required. The Isolation of Trans-acting Mutations The MC4100-derived bacterial strain SR4245 and the BW25113 derivative SR7917 were used as parental strains to isolate Tn10 insertion mutants. Each of these ...
3. Why is MacConkey agar selective as well as differential? 4. Why is blood agar useful as a prim Primary media for isolation of microorganisms: 1. Define a differential medium and discuss its purpose. 2. Define a selective medium and describe its uses....