LDS Lithium dodecylsulfate MES 4-morpholineethanesulfonic acid MOPS 4-morpholinepropanesulfonic acid PAGE polyacrylamide gel electrophoresis SEC size exclusion chromatography Tris 2-Amino-2-(hydroxymethyl)-1,3–propanediol TX–100 Triton X100 Keywords Thyroid Sodium/iodide symporter Membrane protein Eukaryote ...
Samples were then lyophilized, resuspended and boiled in 40 µL 1X lithium dodecyl sulfate (LDS)-phenol red sample buffer (26 mM Tris–HCl, 35 mM Trizma base, 0.5% LDS, 2.5% glycerol, 0.125 mM EDTA, 0.04 mM phenol red). For Western blotting, 10 µL and 4 µL ID and IP ...
sodium resinate sodium saccharin anhy sodium silicate water sodium stearoyl oat p sodium sulfate d sodium sulfatedecahyd sodium sulfosalicylat sodium tannate sodium tetra sulfonat sodium thiamylal sodium thioarseniate sodium undecanoate sodium vapor discharg sodium-dependent carr sodium-hydroxidemetho sod...
Isavuconazonium sulfate is awater-soluble prodrug, which is rapidly hydrolyzed by esterases(mainly butylcholinesterase) in plasma into the active moietyisavuconazole (BAL-4815) and an inactive cleavage product(BAL-8728). Isavuconazole inhibits cytochrome P450 (CYP)-dependent enzyme lanosterol 14-a...
PDE6D:KRAS peptide:compound-1 crystals were obtained under a condition with 0.2 M lithium sulfate, 0.1 M Tris of pH 8.5, and 20% PEG 3000 at 293 K with 10 mg mL–1 PDE6D, 1 mM KRAS peptide, and 8 mM compound-1. The crystals were flash-frozen in liquid nitrogen in the reservoir...
Finally, RNAs and proteins bound to the beads were eluted using a lithium dodecyl sulfate sample buffer (Invitrogen). To isolate RNA covalently cross-linked to proteins, the samples were subjected to SDS-PAGE in a Novex NuPAGE Bis-Tris gel (Invitrogen), and the separated proteins were ...
lithium sulfate and 0.1 M Tris HCl pH 8.5 using the sitting drop vapor diffusion (crystals appeared after 17 days of incubation at 18 °C). The crystallization drop was equilibrated against a 70 μL reservoir solution of same the precipitant. Crystals were cryo-protected by quick-...
Aluminium hydroxide gels can be dehydrated (e.g. using water-miscible non-aqueous solvents like ethanol) to form an amorphous aluminium hydroxide powder, which is readily soluble in acids. Heating converts it to activated aluminas, which are used as desiccants, adsorbent in gas purification, and...
These crystals were obtained using sitting drops at 20 °C in 0.5 m ammonium sulfate, 1 m lithium sulfate, and 0.1 m tri-sodium citrate at pH 5.6 (citrate removes Fe(III) from the protein) from a screen set-up with a crystallization robot using 100 nl of protein and 100 nl of ...
Samples were mixed with a non-reducing loading buffer containing 4% lithium dodecyl sulfate (LDS), heated at 95 °C for 10 min, and resolved on 3% stacking and 12% separating SDS-PAGE. The monomer, oligomer, and aggregate forms of p62 were detected with a mixture of p62 and myc ...