All the bacteria were grown at 37 °C on LB agar plates or in LB liquid medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin. Yeast strain AH109 was used for yeast two-hybrid assay. For yeast complementation assays, S. cerevisiae BY4741 and GRA00743 strains ...
pseudomallei samples from the northeast Thailand collection were cultured on Ashdown, selective agar plates and confirmed the species using latex agglutination test and matrix-laser absorption ionisation mass spectrometry (MALDI-TOF MS). A single colony from Ashdown agar plate was subjected to culture ...
Extracts (200 μg/mL) of these two endophytes also showed selective cytotoxicity action against murine B16-F10 melanoma cells. However, the CMRP4852 extract also affected the density of normal cells. Due to these results, the crude extract of isolate CMRP4860 Verrucosispora sp., which was ...
There was a significant differential between the breast cancer tissues and the adjacent non-cancer tissues, which corresponded to the protein levels in the tissues. In our analysis, high Bmi-1 expression showed an obvious correlation with larger tumor size, lymph node involvement, organ metastasis ...
Five Amino Acids Are Responsible for the Differential Activities of Mouse and Beaver SIRT6 in Stimulating DSB Repair As the activity of SIRT6 in promoting DNA DSB repair is higher in long-lived rodents, we set out to determine which amino acids (aa) are responsible for the differential SIRT6...
Even when strain 33676 expresses the FljB flagellar subunit, instead of FliC expressed by strains SL1344 and YU39, the motility of these three strains was similar on LB plates containing soft agar (data not shown), which supports that a difference in motility is not involved in the invasion...
In terrestrial ecosystems, plant leaves provide the largest biological habitat for highly diverse microbial communities, known as the phyllosphere microbiota. However, the underlying mechanisms of host-driven assembly of these ubiquitous communities rema
Plant roots integrate environmental signals with development using exquisite spatiotemporal control. This is apparent in the deposition of suberin, an apoplastic diffusion barrier, which regulates flow of water, solutes and gases, and is environmentally
Serial dilutions were plated on LB agar for cfu enumeration. Strains were distinguished by differential counting or replica plating on antibiotic-supplemented plates. For each mouse, the CI was calculated by dividing the output ratio (i.e. strain a versus strain b) divided by the input ratio....
ISMs were connected to a high-impedance electrometer (FD 223, WPI, UK), and the circuit was closed by a 1% agar, 1 m KCl bridge. The non-selective barrel was filled with 1 m KCl. α(Na+)i was calculated according to (Na+)i = (Na+)ref× 10exp(VNa − Vm)/S, where (Na+...