Screening of placenta and NT2 cDNA libraries enabled us to obtain the 1.5-kb full-length transcript, which shows a 426-bp open reading frame. Since the resulting 142-amino-acid peptide has a single putative transmembrane domain and a weak but suggestive homology with KCNE1 (minK), a protein...
We monitored cytosolic ATP levels after blocking both the cyanide-sensitive and cyanide-insensitive electron transfer pathways, by treating plants with both KCN and salicylhydroxamic acid (SHAM), inhibitors of the Complex IV and the alternative oxidase (AOX), respectively. After this treatment, the ba...
that ATP may act as a signaling molecule in the white matter and OLs express P2X/P2Y receptors37,38,39,40. The broad-spectrum, nonselective P2X/P2Y receptor antagonists pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS; 50 µM) and suramin (50 µM) reduced the Ca2...
What is the purpose of using a strong acid like sulphuric acid in fluorescence spectroscopy? What do we mean by the equivalence point in a titration? Why does chromatography work? The color change of the phenolphthalein, the indicator, at an end point can be explained in terms of which of ...
{eq}{/eq} KCN is a salt of weak acid (HCN) and strong base (KOH). Therefore, pH of its solution is given by : $$\displaystyle pH = \frac{1}{2}(pKw +... See full answer below.Become a member and unlock all Study Answers Start today. Try it now Create an account Ask...
(Supplementary Fig.2). The frameshift mutation leads to an ~90% reduction incfap20transcript abundance (Fig.2c), presumably due to nonsense-mediated decay24,25,26. These data are consistent withcfap20ua5025being a null allele or strong hypomorph, and we hereafter refer to the mutants asc...
35,58 From our previous work, we find that gene expression for most Kir channels increases in OPCs with age, but the most prominent change is in Kir2.1,21 which is a strong inward rectifier.59 Now, we have shown that Kir2.1 functionally contributes to the age-related increase in inward ...
1. These results establish that qoxABCD encodes a cyanide-sensitive, a-type reductase. TABLE 1 . Respiratory activities of S. aureus terminal oxidase assembly mutants StrainTerminal oxidase(s) presentMembrane respiratory activity After addition of 100 µM KCN (%)Relative to that of WT WT aa3...
Write the conjugate acid of a Grignard reagent, and explain why Grignard reagents are strong bases. Explain why a tertiary carbon is more stable than a methyl carbocation. How would one use a Grignard-based synthesis to accomplish the following transformation: benzyl bromide (PhCH2Br) to 3-phen...
To further explore the arrhythmogenic potential of atrial and pacemaker-like CMs, we developed a bioreactor differentiation protocol to enrich for these subpopulations. By modifying our standard protocol to activate the retinoic acid (RA) signaling pathway from day 2 to day 6 (Extended Data Figs.1a...