Height of viable epidermis (F) and cornified layers (G) in H&E stained Ctrl and Myh9 shRNA knockdown organotypics. 2 organotypics per line, two independent Myh9 shRNA knockdown lines, >3 measurements per FOV, Welch’s t test, *p ≤ 0.05. H Ctrl and Myh9 shRNA knockdown ...
The proportion of viable progeny is the number of L4/adults divided by the number of dead eggs/L1. Cell synchronization in mitosis To enrich cells in mitosis, HeLa or DLD-1 cells were seeded on 0.01% Poly-L-Lysine (Sigma-Aldrich, P8920)-coated coverslips and incubated with 10 μM MG...
Direct killing of sporozoites in the 3D cytotoxicity assay could not completely explain in vivo protection, since 30%–70% of sporozoites were still viable after short preincubation with hmAbs in Matrigel (Figure S6D). These results indicate that migration through 3D substrates (e.g., Matrigel ...
Frise, V. Kaynig, M. Longair, T. Pietzsch, et al. Fiji: an open-source platform for biological-image analysis Nat Methods, 9 (2012), pp. 676-682 CrossrefView in ScopusGoogle Scholar 42 S. Solier, Y. Pommier The nuclear gamma-H2AX apoptotic ring: implications for cancers and ...
3C) and also led to a reduction of viable offspring by about 20%, suggesting a systemic effect of HSP-17 depletion on the animal (Fig. 3D). Next, we assessed how the animals cope with prolonged heat shock (6 h at 35 °C) conditions that severely challenge protein folding. Notably, ...