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To determine whether JEV can induce degranulation of MCs directly without the influence of bystander brain cells, we exposed mouse bone marrow-derived MCs (BMMCs) to a live-attenuated strain of JEV (SA 14-14-2). In contrast to Nakayama, the vaccine strain SA 14-14-2 is not lethal in ...
Our findings in this study are important for validation, evaluation and quality control study of live attenuated flaviviruses vaccines and show that Vero cells are a suitable substrate for the production of a safe and stable live-attenuated JEV vaccine....
Japanese encephalitis virus (JEV) is a leading cause of viral encephalitis. However, the mechanisms of JEV penetration of the blood-brain-barrier (BBB) remain poorly understood. Mast cells (MCs) are granulated innate immune sentinels located perivascular
Live-attenuated vaccines (LAVs) represent a promising approach for flavivirus vaccine development. In the present study, we demonstrated a method for generating flavivirus LAVs based on breaking spatially and temporally regulated C-prM cleavage to disturb the viral assembly process, using an avian ...
JEV is a member of the genus Flavivirus, family Flaviviridae [1]. Like other flaviviruses, the JEV genome is a single-stranded positive-sense RNA of approximately 11 kb in length. It is capped at its 59 end and has a single open reading frame (ORF) that encodes a polyprotein...
Although its precise function remains unknown, the specific deletion of NS1′ results in attenuation of neurovirulence of both WNV and JEV [9, 10]. NS1 is expressed on the surface of cells through at least two mechanisms: (a) soluble NS1 binds back to the plasma membrane of uninfected and...
Although its precise function remains unknown, the specific deletion of NS1′ results in attenuation of neurovirulence of both WNV and JEV [9, 10]. NS1 is expressed on the surface of cells through at least two mechanisms: (a) soluble NS1 binds back to the plasma membrane of uninfected and...
A recent study investigating Japanese encephalitis virus (JEV) entry into C6/36 cells also bears on our findings (Nawa et al., 2007). The results of this study suggest that: (1) JEV can enter C6/36 cells by fusion at the cell surface, resulting in a productive infection even in the ...
Lastly, we generated a vaccine candidate that included the last 22 amino acids of the SP of Japanese Encephalitis Virus (JEV, score 0.931), since this sequence has been used successfully to target proteins for secretion18. Once constructs containing the desired ZIKV antigens were generated (Fig....