Slices were placed under an upright microscope fitted for epifluorescence (Olympus, Tokyo, Japan) and constantly perfused (1.5 ml min−1) with warm (~30 °C) aCSF. mCherry-expressing RP3V neurons were first visualized by brief fluorescence illumination and subsequently approached using inf...
000 females, 59 males (Table 2). Nine individually counted populations yielded a maximum of 200 and a minimum of nine adults/population (patch). The mean sex ratio across 75 adult-emergence days averaged 0.036 (SD = 0.14; N = 75), clearly all highly significantly different to...
Norinchukin wants to sell off US securities (who can blame them?), and the USA puts Japan on a currency watchlist, and there can be little doubt that the move is at least in part and to some degree a response to the Japanese bank's decision. But the real watchlist, and the real les...
The PCR system of bacterial-amoA (B-amoA) and archaeal-amoA (A-amoA) included 6.4 μL of sterile water, 10 μL of SYBR Premix (TaKaRa, Japan), 1.6 μL of the forward/reverse primers and 2 μL of DNA. The PCR system of COM-amoA included 6.4 μL of sterile water, ...
a日本的风景非常美丽。他们从不滥伐树木,以至于他们的山地非常郁郁葱葱;湖水,河水,江流十分清澈。这一点我们应该向他们学习。 Japan's scenery is extremely beautiful.Their ever not denudation trees, their mountainous region is extremely green and luxuriant; The lake water, the river water, the river curren...
The Book of Five Rings,by Miyamoto Musashi, 1645 Japan. The Old Man and the Sea,by Ernest Hemingway, 1952 Cuba Theory of Game Attraction isn’t a choice, by David DeAngelo 2004 USA The Selfish Gene,by Richard Dawkins, 1976 USA.
In fact, although through the years the truth has been perverted to show second and third members on re-issue covers, there are hundreds of examples of the original 5 members on covers from, Japan, Italy, England etc. Plus, our names are listed inside the first album. Any questions?
Japan has 90 – & building 45 more… Total 135, China has 2,363 – & building 1,171 more… Total = 3,534. That’s 5,615 projected coal powered plants in just 8 countries. USA has 15 – & building 0 more… Total = 15.
These amplified gene fragments were cloned into pCRII vectors (TOPO-TA cloning, Invitrogen, Waltham, MA USA) that were used in the transformation of chemically competent E. coli (One Shot® TOP10). Plasmids with correct inserts were sequenced and used for RNA probe synthesis. The identity of...
A cycle of bloody repression and armed rebellion was thus set in motion and would last for a long time thereafter. The most significant case is the massacre of al-Ḥusayn, beloved grandson of Muḥammad and son of ‘Alī, and almost all of his family, by order of the second ...