The histone-like nucleoid structuring (H-NS) protein is a DNA binding factor, found in gammaproteobacteria, with functional equivalents in diverse microbes. Universally, such proteins are understood to silence transcription of horizontally acquired genes
1 ml of cell culture was lysed, pelleted and then resuspended in Nuclei lysis solution (Promega). The cell resuspension was added to a tube containing lysing matrix B (MP Biologicals) and milled three times with intermittent incubation on ice. Phenol-chloroform-isoamyl alcohol was ...
Odours (n = 7; heptanone, isoamyl acetate, limonen, ethyl valerate, pentanol, propyl butyrate and nonane; Sigma-Aldrich, St. Louis, MO) were diluted 1 × 10−3 in mineral oil and applied to a cotton-applicator stick. To prevent contact of the liquid odor with the animal, ...
Isoamyl acetate Banana Cinnamic aldehyde Cinnamon Ethyl propionate Fruity Limonene Orange Ethyl-(E, Z)-2,4-decadienoate Pear Allyl hexanoate Pineapple Ethyl maltol Sugar, Cotton candy Methyl salicylate Wintergreen Benzaldehyde Bitter almond The compounds used to produce artificial flavors are almost identic...
The specificity of TbHMIT (and likewise other trypanosomatid HMITs) could potentially be exploited to design toxic myo-inositol analogs as trypanocidal drugs. In addition, the strict dependence of T. brucei from exogenous myo-inositol validates TbHMIT as a potential drug target. It has been shown...
This suggests that not all increases in R-loops might be necessarily toxic in sen1-3 cells. One possibility is that different mutations might lead to dissimilar levels or distinct biochemical features of the R-loops. Moreover, different genetic backgrounds might lead to the accumulation of DNA:...
ISOAMYL ACETATEALCOHOLACETYLTRANSFERASESACCHAROMYCES-CEREVISIAECELLOBIOSE DEHYDROGENASESEXOGENOUS GLYCINEBETAINEMOLYBDENUM ENZYMESMOLECULAR-CLONINGSTRESSED TOMATOPhlebiopsis gigantea has been routinely used as the biological control agent for the conifer pathogen Heterobasidion annosum sensu lato, but the actual mechanism...
AquaticToxicology55(2001)61–73.elsevier/locate/aquatoxInductionofapoptosisinthebluemusselMytilusgallopro6incialisbytri-n-butyltinchlorideMilenaMic..
50 µg of RNase A were added and the tubes were incubated at 37 °C for 30 min. Another step of phenol: chloroform: isoamyl alcohol extraction was followed by overnight precipitation at −20 °C in the presence of 3 M sodium acetate (pH ± 5), ethanol 96% and gl...
Examples of such radicals include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isoamyl, hexyl and the like. Even more preferred are lower alkyl radicals having one or two carbon atoms. The term “alkylenyl” or “alkylene” embraces bridging divalent...