A DNA fragment encoding Top2 frompFlc1-Top2(DGRC RE49802) was inserted into thepUAST-attBvector for making thepUAST-attB-Top2construct. According to DGRC,Top2 cDNAhas a deletion mutation in 304–364, point nonsense mutation; stop codon in 3802–3601. However, our analysis detected a deletio...
Results: Using a PCR-based scanning method, here we have tested the hypothesis that jal might result from insertion of a transposable element in or near the Gata3 gene. We show that the jal mutation is specifically associated with an intracisternal A particle (IAP) element of the I1 subtype...
Genetic lesion of YFT1 allele in yft1 is attributed to a 573 bp DNA fragment (IF573) insertion at 1,200 bp downstream of the transcription start site. Transcriptomic analysis revealed that YFT1 lesion resulted in 5,053 differentially expressed genes (DEGs) in yft1 pericarp compared with the...
7a–c) produced an anemia phenotype that mirrors the global loss of Hba1 through the insertion of a neomycin resistance cassette20 (Supplementary Fig. 7d–f) with significant defects in blood hemoglobin and hematocrit but with no statistically significant change in total RBCs. Fig. 3: Creation ...
The Drosophila nub gene was originally identified as a viable mutation, nub1, caused by the insertion of a retrotransposon in the promoter region [19, 20] just upstream of the first exon of the nub-RD transcription unit (Additional file 1). The nub gene has been extensively studied for ...
The PCR method we employed for Hsp83 gene-specific small fragment amplification (see Materials and Methods) is proven to be highly sensitive in detecting any insertion/deletion mutation. After having screened about 4500 flies from all 42 populations, we did not find any insertion polymorphism in ...
Here we show that animals harbouring a transposon insertion in the center of the complex nucleoporin98-96 (nup98-96) locus had severe defects in the early steps of this developmental program, ultimately leading to germ cell loss and sterility. A phenotypic analysis indicated that flies carrying...
The new glabrous mutation in cucumber was controlled by a single recessive locuscsgl3, which was phenotypically and genetically distinct from two previously reported glabrous mutantscsgl1andcsgl2. The glabrous phenotype incsgl3was due to insertion of an autonomous, active, class I transposable element...
Screening a library ofRLKT-DNA insertion mutants, we identified SALK_008585 (alr1-1) displaying remarkably reduced Al resistance (Fig.1a, b). Whilst ALR1 is known to be required for root growth,28the morphological development ofalr1-1appears largely identical to that of wild type (WT) (Sup...
In addition to its expression level, we also compared the entire coding region ofHoxd13(1,017 bp) between ecotypes. Although mammalian Hox gene sequences are highly conserved49, we found thatHoxd13had a 3 bp insertion at amino acid position 109 in the disordered N-terminal region of ...