"Heparin" has been and is still used with four different meanings, depending on the author: (1) any heat-stable naturally occurring anticoagulant activity (neutralized by protamine, etc.); (2) a sulfated mucopolysaccharide present in tissues, highly metachromatic, with a high critical electrolyte ...
Fibronectin-coated (356009, Corning Incorporated, USA) dishes were used to culture the cells. Endothelial growth medium (EGM), consisting of endothelial basal medium (EBM) supplemented with human recombinant epidermal growth factor (EGF), EndoCGS-Heparin (PeloBiotech, Germany), 8% fetal calf serum...
Overall, structure–activity relationships that would help establish how CPP composition and structure influence membrane translocation and cell entry are still needed. The HIV TAT peptide is a prototypical arginine-rich CPP that has been widely used as a delivery vector5,6. For instance, TAT has...
Heparin is an intracellular product of vertebrate mast cell currently used as exogenous anticoagulant. Despite of the potent biological activities of exogenous heparin, its physiological function has not been clearly established yet. Here, a heparin with similar structure and anticoagulant properties to th...
BACKGROUND: Low molecular weight heparins (LMWHs) have been cautiously used in patients with chronic kidney disease (CKD) due to fear of accumulation. Dalteparin, however, has shown minimal tendency to accumulate in patients with CKD and may be safe to use in this patient population. OBJECTIVE...
Low-molecular-weight heparin therapy may also find a role perioperatively and in selected patients, notably those with warfarin intolerance, as a replacement for warfarin following cardioversion. Controlled clinical studies are still required, however, to establish a firm, evidence-based foundation for ...
We tested allylamine (AAM), DAP, heptylamine (HA), acrylic acid (AAC), octadiene (OD), and AAM with a layer of heparin adsorbed (AAM-H). The optimized plasma parameters for the high retention of functional groups on the generated plasma polymer films are listed in Table S1. These ...
Blood from healthy volunteers for the in vitro and ex vivo studies was used on the same day. Latter studies were approved by the ethics committee of the University of Lübeck (09–140). 2.2. Cytokines in peripheral blood cells 1 × 106 cells from heparin-NH4-treated patient/control ...
In both humans and mice, vitamin B12is used as a cofactor by only two enzymes: methionine synthase (MS) and methylmalonyl-CoA mutase (MUT)26. MS uses B12as a cofactor to regenerate methionine (Met) from homocysteine (Hcy), forming an integral part of one-carbon (1C) metabolism (Fig.2a...
A large body of evidence has shown that binding of heparin or heparan sulfates (HS)—in particular heparan sulfate proteoglycans (HSPGs)—stabilizes the FGF2 structure and function via the formation of a ternary complex composed of FGF ligand, FGF receptor and HSPGs15,16,17. In addition to ...