SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance. Nature 520, 363–367 (2015). Article ADS CAS PubMed PubMed Central Google Scholar Carlisle, A. E. et al. Selenium detoxification is required for cancer-cell survival. Nat. Metab. 2, 603–611 ...
Mussel calcitonin peptide also seems to regulate mantle enzyme activity and decreased mantle acid phosphatases activity (AP)in vitro. APs are an ancient group of metalloenzymes that are poorly characterized in bivalves. Because of their insensitivity to tartrate inhibition, they are also known as ta...
Galactinol synthase (GS, UDP-α-D-galactose:1L-myo-inositol-1-O-α-D-galactopyranosyltransferase) is a key enzyme in the biosynthetic pathway of the raffinose family of oligosaccharides. The subcellular location of GS was studied in the parenchyma of stachyose-storing tubers of Japanese artichoke...
Efforts to engineer glycine betaine accumulation in fragrant rice need to ensure the pool of GABald is maintained at levels which result in the formation of detectable levels of 2AP. This could be achieved by the use of the codA gene which produces an enzyme that converts choline directly to...
Tumor necrosis factor α converting enzyme (ADAM17) TAILS: Terminal amine isotopic labeling of substrates TBS: Tris-buffered saline TCA: Trichloroacetic acid TMA: Tissue microarray TPS: Total protein stain V5B2: Monoclonal antibody-directed against human shed PrP (PrPY226) WB: Western bl...
Following completion of the PAGE run, samples were transferred to Immobilon FL PVDF membrane (Millipore; Darmstadt, Germany) using Towbin buffer (192 mM glycine, 25 mM Tris). To optimize the transfer of CSF high and low molecular weight proteins, we used a ramped overnight transfer strategy, ...
The standard reaction mixture (0.52 ml) contained 70 mM glycylglycine-NaOH (pH 7.1), 1 mM DTT, 0.20 mM NADH, 0.52 mg (130 IU) of yeast alcohol dehydrogenase, 220 nmol of lysoplasmalogen substrate, and between 1 and 20 μg of LpYhhN enzyme, depending on the specific activity of the...
the answer to which are the methylated vitamins and other nutrients is obvious because the word “methyl” is in the nutrient’s name, such as 5-methyltetrahydrofolate (5MTHF; active folate), methylcobalamin (active B12), dimethylglycine (DMG; derivative of the amino acid glycine), and trimeth...
Cultured bovine pulmonary artery endothelial cells contain a second peptidyl dipeptidase, distinct from angiotensin-converting enzyme, present in an inactive form associated with a non-dialyzable inhibitor. Partial purification by glycine affinity chromatography separates enzyme from inhibitor to yield a prep...
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden Article Open access 02 July 2022 The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2 in the yeast Komagataella phaffii Articl...