Answer to: The monomer that makes up polysaccharides is: a) amino acids b) glucose c) fatty acids d) nucleotides e) glycerol By signing up, you'll...
Then, the supernatant was removed, cells were washed twice with ice-cold PBS and harvested by scraping directly into lysis buffer (100 mM NaCl, 50 mM HEPES-NaOH pH 7.9, 1 mM EDTA, 5% glycerol, 0.05% NP-40, 0.1% SDS, proteases and phosphatases inhibitors − 10 mM sodium fluoride (...
The inactivation occurred at a much slower rate than glycerol and diol dehydratases, with a half-life for inactivation of ∼170 min (28). However, in the absence of substrate the enzyme was stable (28). These precedents suggested that MeaB might play a role in protection or reactivation ...
b BrlR monomer. Three domains are colored as the corresponding monomer in a. c The stacked BrlR dimer from apo-BrlR, two dimers form a homotetramer. d Two monomers of BrlR–c-di-GMP complex in an asymmetric unit. One monomer is shown as in b, one is shown in gray. Sticks diagram ...
Merz A,KnochelT,Jansonius JN. The hyperthermostable indoleglycerol phosphate synthase from thermotoga maritime is destabilized by mutational disruption of two solvent-exposed salt bridges[J].Journal of Molecular Biology,1999,(4):753-763.doi:10.1006/jmbi.1999.2709....
centrifugation and lysed in a lysis buffer (50 mM Tris-HCl, pH 7.4, 200 mM NaCl, 5% glycerol, 1 mM EDTA, 14 mM 2-mercaptoethanol, and 1 mM PMSF) using a sonicator. The recombinant protein was purified by affinity chromatography using Streptactin Beads 4FF (Smart-Lifesciences)...
Characterization of the prepolymer and gel of biocompatible poly(xylitol sebacate) in comparison with poly(glycerol sebacate) using a combination of mass s... Atmospheric pressure chemical ionization (negative ion) and electrospray ionization (positive and negative ion) mass spectrometry and nuclear magne...
Glycerol gradient ultracentrifugation analysis showed that only p54 sedimented as a monomer, while other derivatives were in a homodimeric conformation. We proposed a model to explain the monomeric conformation of catalytically active p54 derivative carrying additional 21-residues long β1′β2′ motif ...
In one method of effecting the process of the invention, a reaction product from the lactone and a molar excess of a monohydric alcohol is reacted with an epoxide, the resultant oxycarboxy monomer being reacted with an excess of the polyfunctional compound, many suitable alcohols, e.g. ...
The eluate was concentrated by around 50-fold using a Vivaspin20 ultrafiltration unit (100 K MWCO, Sartorius) concomitant with an exchange to STO500 buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 2 mM MgCl2, 0.5 mM tris(2-carboxyethyl)phosphine (TCEP), 10% glycerol, 0.1%...