Moreover, under the same conditions, either -SNAP, other proteins apparently unrelated to vesicular transport (glyceraldehyde-3-phosphate dehydrogenase or lactic dehydrogenase) or even 3 mM magnesium ions can also cause lipid mixing. In contrast, neither NSF nor the other proteins nor magnesium had ...
Sugahara T, Shirahata S, Akiyohsi K, Isobe T, Okuyama T & Murakami H (1991) Immunoglobulin production stimulating factor-IIa (IPSF IIα) is a glyceraldehyde-3-phosphate dehydrogenase like protein. Cytotechnology 6: 115–120. PubMedCASGoogle Scholar Sugahara T, Sasaki T & Murakami H (1994)...
Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells. Immunol Lett. 2015;167(2):116–24. Article CAS PubMed Google Scholar Verderio C, Muzio L, Turola E, Bergami A, Novellino L, Ruffini F, et al. Myeloid microvesicles are a ...
Genomic imprinting is observed in endosperm, a placenta-like seed tissue, where transposable elements (TEs) and repeat-derived small RNAs (sRNAs) mediate epigenetic changes in plants. In imprinting, uniparental gene expression arises due to parent-specific epigenetic marks on one allele but not on ...
Conclusion Taken together, DGUOK mutation F180S leads to a lean phenotype, with lower glucose, insulin, and lipid levels rendering this mouse model not only useful for the study of MDDS forms but also for deciphering mechanisms resulting in a lean phenotype.Similar...
In plastids, IPP is produced via in the ‘DOXP pathway’ from pyruvate and glyceraldehyde-3-phosphate (Fig. 1) [12,13]. The first enzyme in this pathway is 1-deoxyxylulose 5-phosphate (DOXP) synthase (DXS), which is encoded by a gene that has been cloned from pepper (Capsicum ...
Quantitative polymerase chain reaction (qPCR) was performed on a Stratagene MX3000 using incorporation of Sybr® green (Sigma-Aldrich, St. Louis, MO, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was chosen as housekeeping gene for the human cell line MG-63, as its expression was ...
Covalent attachment of 4-hydroxynonenal to glyceraldehyde-3-phosphate dehydrogenase. A possible involvement of intra- and intermolecular cross-linking reaction J. Biol. Chem., 268 (1993), pp. 6388-6393 View PDFView articleView in ScopusGoogle Scholar 41. Friguet B., E.R. Stadtman, L.I. Sz...
(Hess et al., J. Adv. Enzyme Reg. 7:149, 1968; and Holland et al., Biochem. 17:4900, 1978), such as enolase, glyceraldehyde-3-phosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6-phosphate isomerase, 3-phosphoglycerate mutase, pyruvate kinase, ...
. Real-time RT-PCR was performed on these samples using a Roche LightCycler 96 with SYBR Green I Master Mix (Roche Life Science). The primers used to amplify target genes are listed in Table S3. The glyceraldehyde 3-phosphate dehydrogenase gene was used as an internal control. Cell culture...