GSH is mostly considered a non-enzymatic antioxidant that serves for modulating the redox status of protein thiols, detoxification, and direct scavenging activity of oxyradicals. Within the cells, GSH has also the role to buffer the flux of nitric oxide (NO), which in the nervous system is ph...
Glutathione is the main endogenous inhibitor of protein glycationdoi:10.4149/gpb_2016044Sabina GaliniakGrzegorz BartoszIzabela Sadowska-BartoszGen Physiol Biophys
To date, ferroptosis has been thought to be controlled only by the phospholipid hydroperoxide-reducing enzyme glutathione peroxidase 4 (GPX4)3,4 and radical-trapping antioxidants5,6. However, elucidation of the factors that underlie the sensitivity of a given cell type to ferroptosis7 is crucial ...
The effects of glutathione, glutathione sulfonate and S-alkyl derivatives of glutathione on the binding of glutamate and selective ligands of ionotropic N-methyl-D-aspartate (NMDA) and non-NMDA receptors were studied with mouse synaptic membranes. The effects of glutathione and its analogues on 45Ca...
The antioxidant is also found in high-quality protein sources such as grass-fed beef, wild-caught fish, and organic eggs. It’s worth clarifying, Sabat says, that while some foods contain glutathione, it is difficult for the body to absorb it directly from food. Glutathione-containing foods ...
However, AN9 was shown to bind flavonoids using three assays: inhibition of the glutathione S-transferase activity of AN9 toward the common substrate 1-chloro 2,4-dinitrobenzene, equilibrium dialysis, and tryptophan quenching. We conclude that AN9 is a flavonoid-binding protein, and propose ...
In the present work, we searched for a GTPase activating protein (GAP) for Rab4 in 3T3-L1 adipocytes. We used a glutathione-S-transferase (GST)-Rab4 protein which possesses the properties of a small GTPase (ability to bind GDP and GTP and to hydrolyse GTP) and can be isolated in a ...
Under a Creative Commons license open accessGlutathione (GSH) has several important functions in eukaryotic cells, and its intracellular concentration is tightly controlled. Combining mathematical models and 35S labeling, we analyzed Saccharomyces cerevisiae sulfur metabolism. This led us to the observation...
Using a seven-step biochemical fractionation procedure and a drop-out mass spectrometry approach, we show that a previously undescribed biomolecule, S-geranylgeranyl-l-glutathione (GGG), is a potent P2RY8 ligand that is detectable in lymphoid tissues at the nanomolar level. GGG inhibited the ...
The anesthetic halothane is bioactivated by the liver cytochrome P450 system to the reactive intermediate, trifluoroacetyl chloride, which can acylate liver protein. Cytosolic glutathione-S-transferase (GST) was identified as a major target for protein adduct formation in guinea pig liver slices expos...