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Is GCU a good business school? GCU was awarded based on exceeding Society standards and developing outstanding programsto provide additional services to members. Heather Talamante, a student in GCU's Business Management program, won “Top Women in Business – Business Women in Recruiting” from the...
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S: 5’-CCAGGCUCAAUGAUAAUGUTT-3’ AS: 5’-ACAUUAUCAUUGAGCCUGGTT-3’ Human ESCA cell line KYSE-150 and KYSE-450 were cultured in a 37oC CO2 incubator using RPMI 1640 medium supplemented with 10% fetal bovine serum. KYSE-150 and KYSE-450 cells were plated in a six-well plate, and...
AAGAGGAGCATATTGGGAAGA; siSNF2H, 5′-CGCUCUCCAUCGUAUGUAAAATT-3′; SNF2H-2, 5′-CCGGGCAAAUAGAUUCGAGUAUUUA-3′; siDDB2-2, 5′-UCACUGGGCUGAAGUUUAA-3′; siXPC–2, 5′-UAGCAAAUGGCUUCUAUCGAA-3′; siCSB, 5′-GCAGUAACUUCUAAUCGAAUU-3′; and siCSB-2, 5′-GAAGAGUUGUCAGUGAUUA...
UCCACCAAUGUACAGGGCUAUCA-30 50-AUAGCCCUGUACAUUGGUGAAUU-30 50-T þ GAT þAGC þ CCT þ GTA þ CAA þTGC þ TG-30 50-C þ ACA þ AAC þ CAT þ TAT þ GTG þ CTG þ CTA-30 50-T þ GTA þ AAC þ CAT þ GAT þ GTG þ CTG þ CTA-30 50...
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ext, the "counts" for each channel (spectral 3sent to the mainUsCinPg Ualgfoorirthpmrso,ctheescseinntgra.l processing unit (CPU) analyzes the spectral data ithms, the centarnadl dperteorcmeinsesitnhegcuonncietn(trCatPioUn )of e spectral dataeaanchdeldemeteenrt mpreinseenst. Cthome...
The target sequence of siRNA for mouse Rac1 is 5′-CAC CAC UGU CCC AAC ACU C-3′, and for mouse Cdc42 is 5′-GAC UCC UUU CUU GCU UGU U-3′; the scrambled negative control siRNA is 5′-UUC UCC GAA CGU GUC ACG U-3′. Western blot Cultured RAW264.7 cells (3 - 4×106) ...
The targeted sequence is 5′-AAAGAUCUCAUGGGCUUTT-3′. Scrambled-siRNA was used as control. Transient transfection of mTOR-siRNA was carried out using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, MA, USA, 13778075). Immunoblot. Whole-cell lysates or homogenized tissue samples...