Cloning of constructs for GCE-experiments in bacterial cells: The pBAD-Rab1b_Q67A-His6 construct was obtained by restriction cloning using HindIII-HF & NdeI (New England Biolabs) and the templates pBad-His6-TEV-EBFP2 and pMal-Rab1b 3-174 opti Q67A and subsequent insertion of the C-te...
Also note that--data_disk_type=localtells PKB not to allocate a separate disk, but to use whatever comes with the VM. This is useful with AWS instance types that come with local SSDs, or with the GCP--gce_num_local_ssdsflag.
But the irony of teaching it to young people who have read almost nothing except their GCE set texts and Adrian Mole, who know almost nothing about the Bible or classical mythology, who cannot recognize an ill-formed sentence, or recite poetry with any sense of rhythm – the irony of ...
A novel, unique, mercury-free, and user-friendly voltammetric sensor for Ni(II) detection, based on a glassy carbon electrode (GCE) modified with a zeolite(MOR)/graphite(G)/dimethylglyoxime(DMG) composite (MOR/G/DMG-GCE), and a corresponding voltammetric procedure for the highly selective and...
The school is looking to add to its bank of exam invigilators (监考人), who will be employed on a casual basis to watch over students taking both mock(模拟) and actual GCSE / GCE exams, as necessary throughout the school year. Full training will be provided and staff will be contacted...
In it, she commented, “I hear a lot of girls saying to hell with ‘A’ level, ‘O’ level, CSE, and GCE, but it will help get you somewhere in life […] We should have ambition, education, and a career in front of us” (Christie 1974, p. 9). Laura Tisdall suggests that ...
potential of Industry 4.0 Envision a socio-technical solution strategy based on the common understanding that communication and cooperation are mission-critical for the overall success of the deployed information system, the design process, and the final result of the mission, which is the building. ...