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(Invitrogen). After 24 hours of cultivation, cells were harvested and (Renillaluciferase activity / firefly luciferase activity) was determined using a Dual-Luciferase Reporter Assay System (Promega). pGL3-Control encoding firefly luciferase served as a control for the calculation of relative ...
setup, the wt and rcm1 deletion strains were co-transformed with the Renilla luciferase (REN) reporter plasmid pLM161 and firefly luciferase (FF) reporters with or without a PTC20. This generatedco-expressed REN/FF and REN/FFPTC reporter pairs in each strain. The luciferaseassays were ...
tTesh.eTRheNdAa-tSaewqeerxepmreasdseioinntoresults of thgeradpohws nusriengguGlartaepdhPaanddPsRoImSMe p5astohftowgaerne-(rLeaspJoolnlas,iCvAe ,rUelSaAte)d. ngs,ennoensewsiegrneifvicaalnidt.ated using QRT-PCR (Supplementary Figure S3). Columbia/A208 d5rf/A208 (a) (b) Columbia/A281...
Six2, a biological marker related to Wnt signaling pathway, promotes the proliferation, inhibits the apoptosis and maintains the un-differentiation of MM cells. Besides, LiCl is an activator of Wnt signaling pathway. However, the role of LiCl in cellular regulation of MM cells remains unclear, ...
The supernatant was then analyzed for both firefly luciferase activity and renilla luciferase activity according to the manufacturer's protocol. 4.6. Pulse-Chase Experiments Pulse-chase experiments were performed after HEK293T cells transfected with either WT- or N137Q mutant-expressing plasmid for 24...