The aspirin molecule is made up of a benzene ring, a carboxyl group, and an ester. It has both polar and non-polar components. Thus, the affinity of water (very polar) for this compound is not as great as the affinity of water for itself. However, the affinity of ethanol molecules (...
molecule, having a slightly negative charge, while the hydrogen atoms, or the bottom of the molecule, having a slightly positive charge. Ethanol is another example of a polar molecule because the oxygen atoms within the molecule have more electronegativity than the other atoms, resulting in a ...
Cells were harvested, washed with cold 10 mmol/L Na2HPO4/NaH2PO4, pH 7.4, containing 0.15 mol/L NaCl (phosphate-buffered saline [PBS]), fixed in 70% ethanol, and then stored at −20°C until analysis. The fixed cells were centrifuged at 1,200 rpm and washed with cold PBS twice, aft...
The en bloc-stained tissues were dehydrated with graded ethanol and embedded in Epon. Ultrathin sections (72 nm) were collected on G400-Cu grids (Electron Microscopy Sciences) and were examined under a Philips CM10 microscope for conventional transmission EM analysis. For FIB-SEM, as ...
Glucose is a polar molecule; its transport across plasma membranes is facilitated largely via the SLC2 family of 13 glucose transporters (Glut). Brain tissues express 8 Glut isoforms, two of which, namely Glut1 and Glut3, are believed to handle the majority of glucose uptake in the brain [...
3). This assay is based on the fact that the disruption of the glycosyl-enzyme intermediate is the rate-limiting step for rBxTW1-E495A using pNPX as donor14. An acceptor able to speed up this disruption regenerates the ability of the enzyme to catalyze the cleavage of a new molecule ...
Clusters of cells were considered to be a clonogenic colony if >40 cells were present. The colonies were imaged, and colony numbers were counted using ImageJ. For cell cycle assays, MM cells were fixed using 70% ethanol and washed with PBS and treated with propidium iodide (PI) solution (...
The molecule(s) responsible for this barrier has not been identified. It may include lipid molecules of the inner eggshell layer playing a role analogous to that of ascarosides. However, EM images of pod-1(ye11) showed that all three eggshell layers appeared intact, but embryos were ...
and placed in labeled bags. It was then washed thoroughly under tap water to remove any soil particles and adhered epiphytes. The plant parts were cut into 0.5 cm segments and surface sterilized using a five-step process as described19. Briefly, the segments were placed in 70% ethanol for ...
would reduce LD accumulation in iMGs. Indeed, the small-molecule PI3K inhibitor GNE-317 dramatically reduced lipid droplet formation inAPOE4/4iMGs exposed to fAβ quantified by live microscopy andPLIN2immunofluorescence (Extended Data Fig.7c,d). In addition, PI3K inhibition with GNE-317 reversed...