After being washed with ethanol, vessels were embedded in agarose and then in paraffin blocks and sectioned at 5 μm thickness. Paraffin was removed by a Histoclear bath, and sections were rehydrated through a 100%/95%/70% ethanol/water gradient before antigen retrieval in citrate solution (...
Littner Y, Tang N, He M et al. (2013). L1 cell adhesion mol- ecule signaling is inhibited by ethanol in vivo. Alcohol Clin Exp Res 37: 383-389.Littner Y, Tang N, He M, Bearer CF. L1 cell adhesion molecule signaling is inhibited by ethanol in vivo. Alcohol Clin Exp Res 2013;...
The en bloc-stained tissues were dehydrated with graded ethanol and embedded in Epon. Ultrathin sections (72 nm) were collected on G400-Cu grids (Electron Microscopy Sciences) and were examined under a Philips CM10 microscope for conventional transmission EM analysis. For FIB-SEM, as ...
Samples were dehydrated 15 min in a graded series of ethanol (25%, 50%, 75%, 95%, 3 3 100%). Samples were incubated 1 3 15min, then 2 3 10min in propylene oxide and embedded in EMbed- 812 epoxy resin (EMS; EPON/Propylene Oxid...
anti- Adenosine A1 Receptor antibody抗体来源 RabbitADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous ...
Deorphanization of a G protein-coupled receptor for oleoylethanolamide and its use in the discovery of small-molecule hypophagic agents Cell Metab., 3 (2006), pp. 167-175 View PDFView articleGoogle Scholar Overton et al., 2008 H.A. Overton, M.C.T. Fyfe, C. Reynet GPR119, a nove...
The samples were stained with 1% osmium tetroxide and dehydrated using ethanol and polypropylene oxide and embedded using Durcupan resin. Samples were sectioned, mounted on grids, and viewed using a FEI Tecnai 12 transmission electron microscope. HRP-labeled structures were identified and their ...
Chlorophyll content was measured using ethanol based on the protocol of Teng et al. (2016). Proline content was examined using the 5-sulfosalicylic acid method, as described in a previous report [40]. Soluble sugar content was measured by the anthrone sulphuric acid method [45]. Photochemical...
The tissue was washed with 1× PBS-T and then incubated in a solution of 0.06% Thio-S in PBS for 8 min. Then, sections were washed for 1 min in 80% ethanol and 5 min in PBS-T. Sections were then counterstained with DAPI for 8 min, washed with PBS-T and coverslipped....
. Briefly, for each sample, 500 mg of ground homogenized dried flower material was mixed with 40 ml of ethanol, agitated for 10 minutes and centrifuged. The supernatant was collected, and the process was repeated twice more on the pellet. An internal standard consisting of 200 μl ...