Supernatants were further subjected to anionic exchange chromatography using a BioLogic LP system (Bio-Rad, Hercules, CA, USA), 5 mL HitrapQ HP columns (GE Healthcare, Chicago, IL, USA), and a flow rate of 3 mL/min. Then, 10 mL of supernatant was diluted in 200 mL buffer A (20 ...
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