Liver tissue area occupied by ANGPTL8-positive cells was measured by using a high-resolution digital video camera (Nikon DXM 1200; Nikon, Tokyo, Japan) connected to a light microscope (Nikon Eclipse E400) equipped with a Plan Apochromat 20× objective (Nikon). Image analysis procedures were ...
For mutant analysis, a minimum of six wildtype (heterozygous) and six mutant embryos were analyzed. Hybridized embryos were cleared in 80% glycerol and photographed using a Nikon digital still camera (DXM1200) on a Nikon stereomicroscope (SMZ1500). Immunohistochemistry e11.5 and P1 embryos were ...