Why is RNA not a genetic material? Why is RNA-seq "better" than Microarrays? Why is there no RNA in the nucleus? Why does DNA have to be transcribed into RNA? Why is DNA more reliable than RNA? Why is ssDNA unstable and RNA stable?
Each PCR reaction included reverse transcriptase negative controls for testing genomic DNA contamination and a non-template negative control to check for primer dimer. The expression of the ITS sequence (ribosomal operon internal transcribed spacer) was determined during the kinetic sequence as ...
Why is DNA more reliable than RNA? How does the structure of DNA differ from that of RNA? Why does RNA have a low fidelity compared to DNA? Why is DNA stable but RNA is not? How the differences and similarities in the structures of DNA and RNA adapt them to their specif...
RNA Methods Embryos for whole-mount in situ hybridization were fixed and hybridized with DIG-labeled Hsp83 antisense RNA probes, which were detected using either HRP-based tyramide signal amplification (http://www.utoronto.ca/krause) or conventional alkaline phosphatase-based (Bashirullah et al., ...
We also evaluated the effect of biologics, immunosuppressants, glucocorticoids, and 5-ASA on the functionality profile of the ileal virome. The majority of viral functions that were inversely associated with these medications were involved in biological processes related to DNA replication, recombination...
(a) FTX expression was determined by qRT-PCR 48 h after control small interfering RNA (siRNA) or FTX siRNA transfection in U87MG and LN18 glioma cells. (b) Cell numbers; (c) colony formation; and (d) MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell ...
As described in the Materials and Methods section, RNA preparations were treated with saturating concentrations of DNAse I followed by purification over columns to eliminate small amounts of any remaining partially-digested small DNA pieces. This step can also remove a substantial fraction of RNAs ...
The rhizosheath-root system is an adaptive trait of sandy-desert speargrasses in response to unfavourable moisture and nutritional conditions. Under the deserts’ polyextreme conditions, plants interact with edaphic microorganisms that positively affect
When cells are stressed, DNA from energy-producing mitochondria can leak out and drive inflammatory immune responses if not cleared. Cells employ a quality control system called autophagy to specifically degrade damaged components. We discovered that mitochondrial transcription factor A (TFAM)—a protein...
However, fusion was still detected within the telomere-adjacent DNA in the absence of LIG3, suggesting that LIG4-dependent C-NHEJ is intrinsically more error prone at telomeric loci presumably resulting from increased end-resection activity. These data are broadly consistent with the processing of ...