Prior to fluorophore labelling, 50 μL of ammonium bicarbonate (1 M) was added to 950 μL of the 0.2 mg/ml solution of antibody solution to increase the pH, followed by the addition of 6.64 μL of 2.5 mM NHS-AF647 (Thermo Fisher Scientific), dissolved in DMSO, to ...
Buruli ulcer (BU) is an emerging infectious disease that causes disfiguring skin ulcers. The causative agent,Mycobacterium ulcerans, secretes toxin called mycolactone that triggers inflammation and immunopathology. Existing treatments are lengthy and consist of drugs developed for tuberculosis. Here, we ...
(endotoxin < 0.005 EU/ml) was obtained from Hyclone. Axitinib was obtained from Santa Cruz (SC-217679). Tauroursodeoxycholic acid (TUDCA) was from Calbiochem (1180-95-6) and 4-phenylbutyric acid (4-PBA) (P21005), Castanospermine (Cas, C3784), Kifunensine (K1140), and DMSO (D...
HNRNPA2 and HNRNPB1 are two splice isoforms transcribed from the same locus but are traditionally treated as a single protein26. HNRNPB1 is a lower abundance (~5%) N-terminal splice variant of the more highly expressed HNRNPA2 isoform and contains an additional 12 aa encoded by exon 227. HN...
doi:10.2147/DMSO.S268992 12. Masana L, Correig E, Ibarretxe D, et al. Low HDL and high triglycerides predict COVID-19 severity. Sci Rep. 2021;11(1):7217. doi:10.1038/ s41598-021-86747-5 13. Alcántara-Alonso E, Molinar-Ramos F, González-López JA, et al. High triglyceride to ...
Fifty-microliter reactions were supplied with 1 μCi γ-[32P] ATP (Perkin Elmer), 10 μM nonradioactive ATP, 10 μM PI:PS (Invitrogen; PV5122), 1× kinase buffer T (20 mM Tris/HCl [pH7.4], 5 mM MgCl2, 0.5 mM EGTA, and 0.4% Triton X-100), 2 mM DTT, 5 μl DMSO, and ...
doi:10.2147/DMSO.S268992 12. Masana L, Correig E, Ibarretxe D, et al. Low HDL and high triglycerides predict COVID-19 severity. Sci Rep. 2021;11(1):7217. doi:10.1038/ s41598-021-86747-5 13. Alcántara-Alonso E, Molinar-Ramos F, González-López JA, et al. High triglyceride to ...
in female mice with oestrogen receptor β deprivation Daniel Kalinowski 1*, Krystyna Bogus‑Nowakowska 1, Anna Kozłowska 2 & Maciej Równiak 1,3 The amygdala is modulated by dopaminergic and cholinergic neurotransmission, and this modulation is altered in mood disorders....
In phos- (50–200 mM), we did not observe the formation of EPR-active species upon addition of up pared in DMSO containing an equimolar amount of to 0.4 mM 18-Crown K6)O. S2i(mFiigla. r7lyA,1w; estdoicdknsootluotbiosnerovfe KfoOrm2 waatisopnroe-f (EXBON;/•5O0 ...
Examples of those include heavy water4, ethanol5,6, glycerol and sorbitol7, caffeine8,9, camp- tothecin10, hydroxyurea11, ATP analogues12, cycloheximide13, DMSO14,15, or TBZ16. New sensitive alleles may also provide additional advantages, such as when studying complex genetic interactions. In...