(P < 0.005) change in the levels of 320 transcripts and 33 metabolites due to dietary selenium insufficiency. Selenium deficiency, as determined by integrated transcriptomic and metabolomic analyses, was found to primarily dysregulate one-carbon metabolism, including the folate and methionine cycle, in...
In summary, we report the discovery of triptonide as a potent and safe nonhormonal male contraceptive agent in mice and nonhuman primates. The success also validates a more general idea for developing male contraceptives. i.e., disabling, rather than “killing”/depleting, all sperm by targetin...
4.6. Pulse-Chase Experiments Pulse-chase experiments were performed after HEK293T cells transfected with either WT- or N137Q mutant-expressing plasmid for 24 h. Cells were starved by replacing culture media with methionine-free DMEM (Invitrogen, Waltham, MA, USA) for 30 min, incubated with ...