In summary, we report the discovery of triptonide as a potent and safe nonhormonal male contraceptive agent in mice and nonhuman primates. The success also validates a more general idea for developing male contraceptives. i.e., disabling, rather than “killing”/depleting, all sperm by targetin...
4.6. Pulse-Chase Experiments Pulse-chase experiments were performed after HEK293T cells transfected with either WT- or N137Q mutant-expressing plasmid for 24 h. Cells were starved by replacing culture media with methionine-free DMEM (Invitrogen, Waltham, MA, USA) for 30 min, incubated with ...