Construct Cloning For overexpression and RNAi constructs, the fragments were amplified by PCR from cDNA of Col-0 using PrimeSTAR HS DNA polymerase (TaKaRa Bio Inc., Kusatsu, Japan) and inserted into a pENTR-1A vector (Invitrogen, Calsbad, CA, USA). Then, the fragments recombined into ...