extract. Lane 4 is the same as lane 3 plus 20× excess unlabeled Probe A; lanes 5 and 6 are the same as lane 3 with 2× and 3× sonicated salmon sperm DNA respectively to inhibit nonspecific protein–probe binding. Lanes 7 and 8 are the same as lane 3 with 2× and 3× poly dI...
While Western blotting (WB) is well-suited to this task, the lack of a validated loading control for CSF WB limits the method's accuracy. New method We investigated the use of total protein (TP) as a CSF WB loading control. Using iodine-based reversible membrane staining, we determined ...
MRI contrast dye risks Don't get contrast dye if you've had anallergic reactionto it in the past or you have severe kidney disease. If you are allergic to shellfish or iodine, or have conditions such asasthma, allergies, anemia, low blood pressure, or sickle cell disease, let your doc...
In contrast above this threshold most late endosomes are permeabilized. Hence, whether a payload is delivered in a given cell tends to be binary: it either escapes from endosomes or not, as opposed to the amount of payload leaking out from endosomes steadily increasing. Our rationale was ...
We began to investigate the mechanisms underlying MFN2 regulation of acetylated α-tubulin by measuring levels and localization of ATAT1 and HDAC6 in Mfn2 KO cells. HDAC6 expression was 3-fold higher in these cells, in contrast to ATAT1 levels, which remained unaffected (Figures S4A and S4B...
MRI contrast dye risks Don't get contrast dye if you've had anallergic reactionto it in the past or you have severe kidney disease. If you are allergic to shellfish or iodine, or have conditions such asasthma, allergies, anemia, low blood pressure, or sickle cell disease, let your doc...
Baseline egg hatch rates were determined in DMSO control wells B48 h after the initial set-up of the assay by the addition of iodine tincture to stop development. Plates having DMSO control wells with hatch rates 470% were assayed on a semi- quantitative gradient of '–' to ' þ þ...
thatit would stop manufacturing its instant cameras and the film they used. However, the millennial fascination with retro technology has led some to attempt to bring back instant cameras in 2017, but professional photographers say these latest attemptsaren’t quite the same as a Polaroids of old...
In contrast, the peroxide-induced slight increase in fluorescence of mitochondria from immotile sperm remained unchanged after addition of either of the Aqp8b inhibitors (Fig. 3c). Protein-G precipitation from SW mitochondrial extracts treated with the Aqp8b-Ab pulled down the phosphorylated and de...
[12,13] However, whether and how aging affects the cardiac autonomic nervous system (ANS) remains to be examined in depth. Previous studies have assessed ANS status in the aged heart by measuring norepinephrine levels and nerve fibers or by metaiodobenzyl guanidine iodine 123 (123I-MIBG) ...