Infants of mothers at high risk of in utero HIV transmission (n = 81) were tested for HIV-1 as soon as possible after birth using point-of-care (PoC) testing to detect total nucleic acid (TNA) PCR on whole blood (GeneXpert Qualitative HIV-1 PCR, Cepheid, Sunnyvale, CA, USA) ...
Latent Mtb infection was diagnosed by QuantiFERON TB Gold in-tube assay (Qiagen, Hilden, Germany, IFN-γ levels >0.35 IU ml−1) and TB disease was diagnosed as sputum-positive positive Xpert MTB/RIF test (Cepheid, Sunnyvale, CA). Whole blood in PAXgene blood RNA tubes (PreAnalytix...
Validity of a point-of-care viral load test for hepatitis B in a low-income setting The recent launch of the first point-of-care Xpert (R) hepatitis B virus (HBV) viral load kit from Cepheid could help to scale up treatment for chronic hep... Woldemedihn, Gezahegn MekonnenRueegg, ...
We performed standard curves on an FDA-approved real-time PCR test for the C. difficile tcd B gene (Xpert C. difficile/Epi, Cepheid) during a prospective comparison of a toxin immunoassay (Meridian Premier), PCR and toxigenic culture. Immunoassay-negative, PCR-positive samples were retested ...
We performed standard curves on an FDA-approved real-time PCR test for the C. difficile tcd B gene (Xpert C. difficile/Epi, Cepheid) during a prospective comparison of a toxin immunoassay (Meridian Premier), PCR and toxigenic culture. Immunoassay-negative, PCR-positive samples were retested ...
以PCR这种相对操作简单的技术平台为例,直到2005年Cepheid(见下图)的第一款全封闭卡盒式自动化PCR检测产品的问世,才实现了qPCR的封闭式自动化。针对NGS这类高度复杂的实验体系,开发自动化平台的难度更是可想而知。 图:Cepheid GeneXpert 挑战并没有吓住思路迪。带着对行业发展趋势的笃定,研发工作启动了。 路线| ...
Design: For each SARS-CoV gene-specific and IPC amplification, a synthetic target DNA was used. PCR reactions were performed using a spectrofluorometric thermal cycler (Cepheid). Materials: The PCR protocols are shown in the following exhibits: ...
2.8. Real-time quantitative PCR Real-time quantitative PCRs were carried out in a Cepheid Smart Cycler Detection system (Cepheid, Sunnyvale, CA). SyBr Green (BioWhittaker Molecular Application, Rockland, ME) and EX Taq real-time quantitative PCR hotstart polymerase were used following the manufactur...
A. et al. Smallpox and pan-orthopox virus detection by real-time 3′-minor groove binder TaqMan assays on the roche LightCycler and the Cepheid smart Cycler platforms. J. Clin. Microbiol. 42, 601–609 (2004). Article CAS PubMed PubMed Central Google Scholar Wang, T. et al. The ...
FDA approved RT-PCR assays included the Cobas 6800 (Roche Diagnostics, Rotkreuz, Switzerland), Abbott Alinity (Abbott Labs, Abbott Park, IL, USA), Cepheid GeneXpert (Cepheid, Sunnyvale, CA, USA), and the Panther Fusion (Hologic, Marlborough, MA, USA). Quantitative PCR data (i.e. Ct ...