Cotyledons were then transferred to a selective plate (MS with 200 mg l−1 cefotaxime, 50 mg l−1 kanamycin). Thirty independent antibiotic-resistant roots were subcloned for each transgenic line for future analyses with cefotaxime+kanamycin for 2 rounds of subcloning and maintained ...
The second-generation cephalosporin cefotetan (96.2% effectiveness) was more effective than the third-generation cephalosporin cefotaxime (69.8%) for the treatment of infections caused by Gram-negative organisms. Vancomycin and teicoplanin, achieving an 838% success rate, were the most suitable ...
After regeneration, the shoots were grown on MS20 media containing cefotaxime (200 µg/ml), vancomycin (200 µg/ml), and kanamycin (100 µg/ml). When the shoots reached sufficient length, cuttings were made and grown in MS20 without antibiotics. After at least two weeks of ...
The β-lactamase activity of GqqA was determined via a disc diffusion antibiotic susceptibility test. Ampicillin 10 µg (AMP), Amoxicillin 10 µg (AML), Cefotaxime 30 µg (CTX 30) and Penicillin G 10 µg (P) sensitivity discs (Thermo Fischer Scientific, Waltham, MA, USA) were in...
After regeneration, the shoots were grown on MS20 media containing cefotaxime (200 µg/ml), vancomycin (200 µg/ml), and kanamycin (100 µg/ml). When the shoots reached sufficient length, cuttings were made and grown in MS20 without antibiotics. After at least two weeks of growing ...
was distinct from NDM-37 due to a supplementary missense mutation, an alteration from Alanine to Valine at position 233. NDM-36's hydrolytic activity toward ampicillin and cefotaxime was superior to that of NDM-37 and NDM-5; in contrast, NDM-37 and NDM-36 exhibited lower activity in ...
The finding of elevation of CSF WBCs and protein demands the use of parenteral agents, such as penicillin G, cefotaxime, or ceftriaxone, for 2 or more weeks. Less obvious is whether to examine the CSF in patients with isolated facial palsy. The cranial nerve involvement in such cases is ...
miningococcal mingitis Meningococcalmeningitis(Epidemiccerebrospinalmeningitis)Huzhiliang,M.D.DepartmentofInfectiousDiseaseThesecondhospitalofnanjing Featuresofmeningitis Causes Infection,drugs,connectivetissuedisorders,tumors Clinicalmanifestations FeverHeadache,nausea,vomiting,confusionMeningealirritation...
(Chen et al.2016; Wang et al.2018a). The research on the pathogenic function of unconventionally secreted proteins inV. dahliaeis still in the initial stages. For example, VdIsc1 can suppress salicylate-mediated innate immunityin plantaby hydrolyzing isochorismate, a precursor of salicylate (Liu ...
The cellophane was transferred to a new PDA solid medium (containing 200 mmol/L cefotaxime sodium and 200 mmol/L hygromycin B) after being co-cultivated at 28 °C for 2 days and removed it after cultivation at 30 °C for 1 day. Cultivation for 6–8 days until growth of the A.niger ...