该研究发现IS200/IS605转座子家族与独特的ncRNA相关,编码可编程RNA引导型核酸酶IscB,IsrB和TnpB(统称为Ω),其中IscB和TnpB可能是CRISPR核酸内切酶Cas9s和Cas12s的祖先。 Fig. 1. IscBs are associated with ncRNAs of unknown function....
CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase (DNA-PK) complex and disrupts the interaction between KU86 and its kinase subunit, leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining (NHEJ) pathway. XCAS9 is a CAS9 variant ...
Protein ubiquitination plays a critical role in protein quality control in response to cellular stress. The excessive accumulation of ubiquitinated conjugates can be detrimental to cells and is recognized as a hallmark of multiple neurodegenerative disea
The majority of the eukaryotic genome is transcribed into noncoding RNAs (ncRNAs), which are important regulators of different nuclear processes by controlling chromatin structure. However, the full extent of ncRNA function has remained elusive. Here we
ISG15 is an interferon-stimulated, ubiquitin-like protein that can conjugate to substrate proteins (ISGylation) to counteract microbial infection, but the underlying mechanisms remain elusive. Here, we use a virus-like particle trapping technology to ide
At present, the hottest and most widely used CRISPR-Pro system is based on Cas9 protein and guide RNA (gRNA). Cas9 contains two active sites: RuvC at the N-terminus and the HNH nuclease domain that is situated in the middle of the protein. These active sites play a coordinated role in...
BS-QCV-240A 定制密闭式矩形石英比色皿,7ml,10mm 植物叶绿体中果糖-1,6-二磷酸醛缩酶(FDA)检测试剂盒 UV板 微量法,FDA Assay Kit,货号:BC2005-100管/96样 Recombinant human MAD2L1BP protein 标准型基因扩增仪 Akt3,Goat IgG anti-Mouse/Rat/Human,WB/IP/ 衣霉素 BIOAMF-2羊水细胞培养-BI羊...
The CRISPR system employs two main components: the Cas9 protein, which acts as a pair of molecular scissors, and a programmable RNA guide, that recognizes specific sequences of DNA. The RNA guide is a single strand that is complimentary to a certain target gene, for example, one that is ...
Duchenne muscular dystrophy (DMD) is a severe hereditary disease caused by a deficiency in the dystrophin protein. The most frequent types of disease-causing mutations in the DMD gene are frameshift deletions of one or more exons. Precision genome editing systems such as CRISPR-Cas9 have shown po...
2.5. CRISPR/Cas9-mediated deletion of trg A clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 method (CRISPR/Cas9) was designed to knock out trg in the T. gondii ME49 ΔHPT:LUC strain (Tobin and Knoll, 2012). A guide sequence (5′-AGTGGATGCGGAGCCTGCTG-...